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Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae

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Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae

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dc.contributor.author Martínez Montañés, Fernando Vicente es_ES
dc.contributor.author Rienzo, Alessandro es_ES
dc.contributor.author Poveda Huertes, Daniel es_ES
dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.contributor.author Proft, Markus Hans es_ES
dc.date.accessioned 2017-03-23T08:09:08Z
dc.date.available 2017-03-23T08:09:08Z
dc.date.issued 2013-05
dc.identifier.issn 1535-9778
dc.identifier.uri http://hdl.handle.net/10251/78985
dc.description.abstract [EN] Mot3 and Rox1 are transcriptional repressors of hypoxic genes. Both factors recently have been found to be involved in the adaptive response to hyperosmotic stress, with an important function in the adjustment of ergosterol biosynthesis. Here, we determine the gene expression profile of a mot3 rox1 double mutant under acute osmostress at the genomic scale in order to identify the target genes affected by both transcription factors upon stress. Unexpectedly, we find a specific subgroup of osmostress-inducible genes to be under positive control of Mot3. These Mot3-activated stress genes also depend on the general stress activators Msn2 and Msn4. We confirm that both Mot3 and Msn4 bind directly to some promoter regions of this gene group. Further-more, osmostress-induced binding of the Msn2 and Msn4 factors to these target promoters is severely affected by the loss of Mot3 function. The genes repressed by Mot3 and Rox1 preferentially encode proteins of the cell wall and plasma membrane. Cell conjugation was the most significantly enriched biological process which was negatively regulated by both factors and by osmotic stress. The mating response was repressed by salt stress dependent on Mot3 and Rox1 function. Taking our findings together, the Mot3 transcriptional regulator has unanticipated diverse functions in the cellular adjustment to osmotic stress, including transcriptional activation and modulation of mating efficiency. es_ES
dc.description.sponsorship This work was supported by grants BFU2008-00271 and BFU2011-23326 from the Ministry of Science and Innovation (Madrid, Spain). F. Martinez-Montanes was supported by an FPI predoctoral fellowship from the Ministry of Science and Innovation (Madrid, Spain).
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation MICINN/BFU 2008-00271 es_ES
dc.relation MICINN/BFU 2011-23326
dc.relation.ispartof Eukaryotic Cell es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Hog1 map kinase es_ES
dc.subject Gene expression es_ES
dc.subject Osmotic stress es_ES
dc.subject Hypoxic genes es_ES
dc.subject Pol II es_ES
dc.subject Yeast es_ES
dc.subject Protein es_ES
dc.subject Binding es_ES
dc.subject Oxygen es_ES
dc.subject Rox1 es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/EC.00037-13.
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Martínez Montañés, FV.; Rienzo, A.; Poveda Huertes, D.; Pascual-Ahuir Giner, MD.; Proft, MH. (2013). Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae. Eukaryotic Cell. 12(5):636-647. doi:10.1128/EC.00037-13 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/ 10.1128/EC.00037-13 es_ES
dc.description.upvformatpinicio 636 es_ES
dc.description.upvformatpfin 647 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 259421 es_ES
dc.identifier.pmid 23435728
dc.identifier.pmcid PMC3647766 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación (MICINN)


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