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A VIN1 GUS::GFP fusion reveals activated sucrose metabolism occurring in interspersed cells during tomato fruit ripening

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A VIN1 GUS::GFP fusion reveals activated sucrose metabolism occurring in interspersed cells during tomato fruit ripening

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dc.contributor.author Hueso Estornell, Leandro es_ES
dc.contributor.author Pons Puig, Clara es_ES
dc.contributor.author Martinez, Alicia es_ES
dc.contributor.author O’Connor, José Enrique es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.date.accessioned 2017-04-03T12:51:57Z
dc.date.available 2017-04-03T12:51:57Z
dc.date.issued 2013-08-15
dc.identifier.issn 0176-1617
dc.identifier.uri http://hdl.handle.net/10251/79396
dc.description.abstract [EN] The tomato is a model for fleshy fruit development and ripening. Here we report on the identification of a novel unique cell autonomous/cellular pattern of expression that was detected in fruits of transgenic tomato lines carrying a GFP GUS driven by the fruit specific vacuolar invertase promoter VIN1. The VIN1 promoter sequence faithfully reproduced the global endogenous VIN expression by conferring a biphasic pattern of expression with a second phase clearly associated to fruit ripening. A closer view revealed a salt and pepper pattern of expression characterized by individual cells exhibiting a range of expression levels (from high to low) surrounded by cells with no expression. This type of pattern was detected across differentfruittissues and celltypes with some preferences for vascular, sub-epidermal layer and the inner part ofthe fruit. Cell ability to show promoter activity wasneither directly associated withoverall ripening – as we find VIN+ and – VIN− cells at all stages of ripening, nor with cell size. Nevertheless the number of cells with active VIN-driven expression increased with ripening and the activity of the VIN promoter seems to be inversely correlated with cell size in VIN+ cells. Gene expression analysis of FACS-sorted VIN+ cells revealed a transcriptionally distinct subpopulation of cells defined by increased expression of genes related to sucrose metabolism, and decreased activity in protein synthesis and chromatin remodeling. This finding suggests that local micro heterogeneity may underlie some aspects (i.e. the futile cycles involving sucrose metabolism) of an otherwise more uniform looking ripening program es_ES
dc.description.sponsorship This work has been supported by the Spanish Ministry of Science and Education (Ramon y Cajal programs and Project BIO2005-01015), the European Commission (program I3P and Project EU-SOL) and the Fundacion Genoma Espana (ESP-SOL project). We thank Dr. Mondher Bouzayen for the EU_TOM1_12K (INRA/INP-ENSAT, France) oligonucleotide microarray slides, Jose Antonio Daros for his help in setting up the protoplast isolation method, Maria Dolores Gomez for her assistance in the microscopy techniques, Asun Fernandez for advice and Rafael Martinez for his skilled care of the tomato plants in the greenhouse.
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject FACS es_ES
dc.subject Invertase es_ES
dc.subject Protoplasts es_ES
dc.subject Ripening es_ES
dc.subject Tomato es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A VIN1 GUS::GFP fusion reveals activated sucrose metabolism occurring in interspersed cells during tomato fruit ripening es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jplph.2013.03.007
dc.relation.projectID info:eu-repo/grantAgreement/MEC//BIO2005-01015/ES/PRODUCCION DE PROTEINAS INMUNOTERAPEUTICAS DE APLICACION ORAL EN FRUTOS DE TOMATE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/268465/EU/Real-time experimental study of pattern selection dynamics in eutectic solidification/
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hueso Estornell, L.; Pons Puig, C.; Martinez, A.; O’connor, JE.; Orzáez Calatayud, DV.; Granell Richart, A. (2013). A VIN1 GUS::GFP fusion reveals activated sucrose metabolism occurring in interspersed cells during tomato fruit ripening. Journal of Plant Physiology. 170(12):1113-1121. https://doi.org/10.1016/j.jplph.2013.03.007 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.jplph.2013.03.007 es_ES
dc.description.upvformatpinicio 1113 es_ES
dc.description.upvformatpfin 1121 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 170 es_ES
dc.description.issue 12 es_ES
dc.relation.senia 256769 es_ES
dc.identifier.pmid 23598179
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder European Commission


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