dc.contributor.author |
Hueso Estornell, Leandro
|
es_ES |
dc.contributor.author |
Pons Puig, Clara
|
es_ES |
dc.contributor.author |
Martinez, Alicia
|
es_ES |
dc.contributor.author |
O’Connor, José Enrique
|
es_ES |
dc.contributor.author |
Orzáez Calatayud, Diego Vicente
|
es_ES |
dc.contributor.author |
Granell Richart, Antonio
|
es_ES |
dc.date.accessioned |
2017-04-03T12:51:57Z |
|
dc.date.available |
2017-04-03T12:51:57Z |
|
dc.date.issued |
2013-08-15 |
|
dc.identifier.issn |
0176-1617 |
|
dc.identifier.uri |
http://hdl.handle.net/10251/79396 |
|
dc.description.abstract |
[EN] The tomato is a model for fleshy fruit development and ripening. Here we report on the identification of
a novel unique cell autonomous/cellular pattern of expression that was detected in fruits of transgenic
tomato lines carrying a GFP GUS driven by the fruit specific vacuolar invertase promoter VIN1. The VIN1
promoter sequence faithfully reproduced the global endogenous VIN expression by conferring a biphasic
pattern of expression with a second phase clearly associated to fruit ripening. A closer view revealed a
salt and pepper pattern of expression characterized by individual cells exhibiting a range of expression
levels (from high to low) surrounded by cells with no expression. This type of pattern was detected across
differentfruittissues and celltypes with some preferences for vascular, sub-epidermal layer and the inner
part ofthe fruit. Cell ability to show promoter activity wasneither directly associated withoverall ripening
– as we find VIN+ and – VIN− cells at all stages of ripening, nor with cell size. Nevertheless the number
of cells with active VIN-driven expression increased with ripening and the activity of the VIN promoter
seems to be inversely correlated with cell size in VIN+ cells. Gene expression analysis of FACS-sorted VIN+
cells revealed a transcriptionally distinct subpopulation of cells defined by increased expression of genes
related to sucrose metabolism, and decreased activity in protein synthesis and chromatin remodeling.
This finding suggests that local micro heterogeneity may underlie some aspects (i.e. the futile cycles
involving sucrose metabolism) of an otherwise more uniform looking ripening program |
es_ES |
dc.description.sponsorship |
This work has been supported by the Spanish Ministry of Science and Education (Ramon y Cajal programs and Project BIO2005-01015), the European Commission (program I3P and Project EU-SOL) and the Fundacion Genoma Espana (ESP-SOL project). We thank Dr. Mondher Bouzayen for the EU_TOM1_12K (INRA/INP-ENSAT, France) oligonucleotide microarray slides, Jose Antonio Daros for his help in setting up the protoplast isolation method, Maria Dolores Gomez for her assistance in the microscopy techniques, Asun Fernandez for advice and Rafael Martinez for his skilled care of the tomato plants in the greenhouse. |
|
dc.language |
Inglés |
es_ES |
dc.publisher |
Elsevier |
es_ES |
dc.relation.ispartof |
Journal of Plant Physiology |
es_ES |
dc.rights |
Reserva de todos los derechos |
es_ES |
dc.subject |
FACS |
es_ES |
dc.subject |
Invertase |
es_ES |
dc.subject |
Protoplasts |
es_ES |
dc.subject |
Ripening |
es_ES |
dc.subject |
Tomato |
es_ES |
dc.subject.classification |
BIOQUIMICA Y BIOLOGIA MOLECULAR |
es_ES |
dc.title |
A VIN1 GUS::GFP fusion reveals activated sucrose metabolism occurring in interspersed cells during tomato fruit ripening |
es_ES |
dc.type |
Artículo |
es_ES |
dc.identifier.doi |
10.1016/j.jplph.2013.03.007 |
|
dc.relation.projectID |
info:eu-repo/grantAgreement/MEC//BIO2005-01015/ES/PRODUCCION DE PROTEINAS INMUNOTERAPEUTICAS DE APLICACION ORAL EN FRUTOS DE TOMATE/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/EC/FP7/268465/EU/Real-time experimental study of pattern selection dynamics in eutectic solidification/ |
|
dc.rights.accessRights |
Cerrado |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes |
es_ES |
dc.description.bibliographicCitation |
Hueso Estornell, L.; Pons Puig, C.; Martinez, A.; O’connor, JE.; Orzáez Calatayud, DV.; Granell Richart, A. (2013). A VIN1 GUS::GFP fusion reveals activated sucrose metabolism occurring in interspersed cells during tomato fruit ripening. Journal of Plant Physiology. 170(12):1113-1121. https://doi.org/10.1016/j.jplph.2013.03.007 |
es_ES |
dc.description.accrualMethod |
S |
es_ES |
dc.relation.publisherversion |
http://dx.doi.org/10.1016/j.jplph.2013.03.007 |
es_ES |
dc.description.upvformatpinicio |
1113 |
es_ES |
dc.description.upvformatpfin |
1121 |
es_ES |
dc.type.version |
info:eu-repo/semantics/publishedVersion |
es_ES |
dc.description.volume |
170 |
es_ES |
dc.description.issue |
12 |
es_ES |
dc.relation.senia |
256769 |
es_ES |
dc.identifier.pmid |
23598179 |
|
dc.contributor.funder |
Ministerio de Educación y Ciencia |
|
dc.contributor.funder |
European Commission |
|