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Comparative proteomic analysis of melon phloem exudates in response to viral infection

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Comparative proteomic analysis of melon phloem exudates in response to viral infection

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dc.contributor.author Serra Soriano, Marta es_ES
dc.contributor.author Navarro Bohigues, José Antonio es_ES
dc.contributor.author Genovés Martínez, Ainhoa es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.date.accessioned 2017-04-04T12:40:03Z
dc.date.available 2017-04-04T12:40:03Z
dc.date.issued 2015-06-21
dc.identifier.issn 1874-3919
dc.identifier.uri http://hdl.handle.net/10251/79445
dc.description.abstract Despite the importance of phloem as an integrative pathway for resource distribution, signaling and plant virus transport little is known about the modifications induced by these pathogens in phloem sap proteome. Only one previous study has actually examined the phloem sap proteome during viral infection using conventional two-dimensional electrophoresis. Since the major limitation of this technique has been its low sensitivity, the authors only identified five phloem proteins with altered abundance. To circumvent this issue we use two-dimensional difference in-gel electrophoresis (2D DIGE) technique, which combined with DeCyder Differential Analysis Software allows a more accurate and sensitive quantitative analysis than with conventional 2D PAGE. We identified 19 different proteins which accumulation in phloem sap was altered during a compatible plant virus infection including redox and hypersensitivity response-related proteins. Therefore, this work would help to understand the basic processes that occur in phloem during plant-virus interaction. (C) 2015 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship This work was funded by grant BIO2014-54862-R from the Spanish Direccion General de Investigacion Cientifica y Tecnica (DGICYT) and the Prometeo Program GV2011/003 from the Generalitat Valenciana. J.A.N. and M.S. are the recipients of a postdoctoral contract and a PhD fellowship from the Ministerio de Educacion y Ciencia of Spain. We thank L. Corachan for her technical assistance. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Spanish Direccion General de Investigacion Cientifica y Tecnica (DGICYT) BIO2014-54862-R es_ES
dc.relation Prometeo Program from the Generalitat Valenciana GV2011/003 es_ES
dc.relation Ministerio de Educacion y Ciencia of Spain es_ES
dc.relation.ispartof Journal of Proteomics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Plant Virus es_ES
dc.subject Phloem es_ES
dc.subject Proteome es_ES
dc.subject Hypersensitive Response es_ES
dc.subject Redox balance es_ES
dc.subject Coat protein es_ES
dc.title Comparative proteomic analysis of melon phloem exudates in response to viral infection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jprot.2015.04.008
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Serra Soriano, M.; Navarro Bohigues, JA.; Genovés Martínez, A.; Pallás Benet, V. (2015). Comparative proteomic analysis of melon phloem exudates in response to viral infection. Journal of Proteomics. 124:11-24. doi:10.1016/j.jprot.2015.04.008 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.jprot.2015.04.008 es_ES
dc.description.upvformatpinicio 11 es_ES
dc.description.upvformatpfin 24 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 124 es_ES
dc.relation.senia 309759 es_ES


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