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Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality

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Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality

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dc.contributor.author Vilchez Olivencia, Maria Carmen es_ES
dc.contributor.author D. Pla es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Sanz, Libia es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.contributor.author Calvete, Juan J. es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.date.accessioned 2017-04-25T16:31:07Z
dc.date.available 2017-04-25T16:31:07Z
dc.date.issued 2016-11
dc.identifier.issn 1095-6433
dc.identifier.uri http://hdl.handle.net/10251/79955
dc.description.abstract [EN] By first time, 2DE protein profile of European eel seminal plasma has been determined. 14 different proteins corresponding to 9 major families were identified in seminal plasma, through hormonal treatment. Some of them play a part in sperm maturation, including carbonic anhydrase which is responsible for modulating the pH of seminal plasma, and warm temperature acclimation protein, which may play an important role in the final maturation of this species, due to the warm temperature of their spawning ground (in the Sargasso Sea). Sperm samples were classified into three motility categories depending on the percentage of motile cells, I: 0-25%, II: 25-50% and 111: >50%. Different protein profiles were observed depending on the sperm motility categories, specifically, with the apolipoproteins and complement C3. Higher numbers of proteins from the apolipoprotein family were registered at lower motilities; whereas the complement C3-like family was higher in the samples with the highest percentage of motile cells. These results suggest that the proteins linked to the transportation of lipids (apolipoprotein) and to the immune system (complement C3) may carry out their functions at different stages of spermatogenesis. Using SDS-PAGE analysis, 13 bands were identified, most of which migrated between 20 to 60 kDa. In the last weeks of treatment significant increases were observed in the percentage of motile spermatozoa, curvilinear velocity and beat cross frequency. This improvement in sperm quality coincided with a higher amount of proteins located at 19 KDa, therefore, this protein could be involved in sperm motility of the European eel. (C) 2016 Published by Elsevier Inc. es_ES
dc.description.sponsorship Funded from the REPRO-TEMP project (Spanish Ministry of Science and Innovation, MICINN; AGL2013-41646-R). and COST Office (COST Action FA1205: AQUAGAMETE). M.C.V. and V.G. have pre- and post-doctoral grants from UPV PAID Programme (2011-S2-02-6521 and 10-14 respectively). Valenciana de Acuicultura, S.A. (Puzol, Spain) supplied the eels used in this study. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation REPRO-TEMP project (Spanish Ministry of Science and Innovation, MICINN) es_ES
dc.relation COST Office (COST Action FA1205: AQUAGAMETE) es_ES
dc.relation UPV PAID Programme es_ES
dc.relation.ispartof Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject CASA system es_ES
dc.subject Proteomics es_ES
dc.subject Sperm motility es_ES
dc.subject LC MS/MS es_ES
dc.subject 2D-electrophoresis es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.cbpa.2016.06.025
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Vilchez Olivencia, MC.; D. Pla; Gallego Albiach, V.; Sanz, L.; Pérez Igualada, LM.; Asturiano Nemesio, JF.; Calvete, JJ.... (2016). Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology. 201:37-45. doi:10.1016/j.cbpa.2016.06.025 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.cbpa.2016.06.025 es_ES
dc.description.upvformatpinicio 37 es_ES
dc.description.upvformatpfin 45 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 201 es_ES
dc.relation.senia 315452 es_ES
dc.identifier.eissn 1531-4332


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