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A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays

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A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays

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Nombre: Gonzalez-ibeas, D. ...
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dc.contributor.author Gonzalez-Ibeas, Daniel es_ES
dc.contributor.author Blanca Postigo, José Miguel es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Truniger, Veronica es_ES
dc.contributor.author Aranda, Miguel A. es_ES
dc.date.accessioned 2017-04-28T10:30:28Z
dc.date.available 2017-04-28T10:30:28Z
dc.date.issued 2012-10
dc.identifier.issn 0735-9640
dc.identifier.uri http://hdl.handle.net/10251/80205
dc.description.abstract [EN] DNA microarrays are two-dimensional arrangements of specific probes deposited on a substrate that have been widely used in gene expression analysis by measuring mRNA accumulation. The use of this type of microarrays involves the synthesis of cDNA, which has to be double stranded (ds) if the microarray probes are of the positive strand. We have used a melon custom-synthesized noncommercial NimbleGen microarray to evaluate a modification of the SMART¿ (switching mechanism at the 5¿ end of the RNA transcript) procedure of ds cDNA synthesis, which differs substantially in its economical cost relative to a widely recommended method based on the nick translation approach. The results suggested that both methods produce cDNA representative of the transcriptome to a similar extent, indicating that the alternative technique provides a cheaper method of ds cDNA synthesis for plant microarray gene expression assays when the RNA starting material is not limiting. © 2012 Springer-Verlag. es_ES
dc.description.sponsorship This work was supported by grant AGL2009-07552/AGR. We thank Mari Carmen Montesinos and Blanca Gosalvez for their technical assistance.
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Plant Molecular Biology Reporter es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Microarray es_ES
dc.subject Double-stranded cDNA es_ES
dc.subject Gene expression es_ES
dc.subject Cucumis es_ES
dc.subject.classification GENETICA es_ES
dc.title A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11105-012-0427-5
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2009-07552/ES/Traduccion De Rnas De Virus De Plantas Independiente De Cap: Analisis Del Modelo Experimental Mnsv%2FMelon Y Aplicaciones Biotecnologicas/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Gonzalez-Ibeas, D.; Blanca Postigo, JM.; Cañizares Sales, J.; Truniger, V.; Aranda, MA. (2012). A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays. Plant Molecular Biology Reporter. 30(5):1276-1282. https://doi.org/10.1007/s11105-012-0427-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s11105-012-0427-5 es_ES
dc.description.upvformatpinicio 1276 es_ES
dc.description.upvformatpfin 1282 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 231898 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación
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