De Panis, DN.; Padró, J.; Furió Tarí, P.; Tarazona Campos, S.; Milla Carmona, PS.; Soto, IM.; Dopazo, H.... (2016). Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila. Molecular Ecology. 25(18):4534-4550. doi:10.1111/mec.13785
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/80336
Title:
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Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
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Author:
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De Panis, Diego N.
Padró, Julián
Furió Tarí, Pedro
Tarazona Campos, Sonia
Milla Carmona, Pablo S.
Soto, Ignacio M.
Dopazo, Hernán
Conesa, Ana
Hasson, Esteban
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UPV Unit:
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Universitat Politècnica de València. Facultad de Administración y Dirección de Empresas - Facultat d'Administració i Direcció d'Empreses
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Issued date:
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Abstract:
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[EN] High-throughput transcriptome studies are breaking new ground to investigate the
responses that organisms deploy in alternative environments. Nevertheless, much
remains to be understood about the genetic basis of ...[+]
[EN] High-throughput transcriptome studies are breaking new ground to investigate the
responses that organisms deploy in alternative environments. Nevertheless, much
remains to be understood about the genetic basis of host plant adaptation. Here, we
investigate genome-wide expression in the fly Drosophila buzzatii raised in different
conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary
rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus
terscheckii. The latter constitutes a harmful host, rich in mescaline and other
related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae
reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids
extracted from the latter. Whole-genome expression profiles were massively modulated
by the rearing environment, mainly by the presence of T. terscheckii alkaloids.
Differentially expressed genes were mainly related to detoxification, oxidation–reduction
and stress response; however, we also found genes involved in development and
neurobiological processes. In conclusion, our study contributes new data onto the role
of transcriptional plasticity in response to alternative rearing environments.
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Subjects:
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Alkaloids
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Environment adaptation
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Mescaline
,
Plasticity
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RNA-Seq
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Copyrigths:
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Cerrado |
Source:
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Molecular Ecology. (issn:
0962-1083
) (eissn:
1365-294X
)
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DOI:
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10.1111/mec.13785
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Publisher:
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Wiley
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Publisher version:
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http://dx.doi.org/10.1111/mec.13785
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Project ID:
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Argentinian grants from Universidad de Buenos Aires (UBACyT 20020130100058BA), CONICET (PIP 11220150100029CO) and ANPCyT (PICT 2795/2010 and 1121/ 2013)
European Regional Development Funds and Marie Curie IRSES Project DEANN (PIRSES-GA-2013-612583)
Spanish MINECO grants (PIB2010AR-00266 and BIO2012-40244)
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Thanks:
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We thank Dr. A. Ruiz for early access to reference genome, S. Szajnman for the invaluable chemistry technical assistance and Lic. J. Vrdoljak for his assistance in DT measurements. This work was supported by Argentinian ...[+]
We thank Dr. A. Ruiz for early access to reference genome, S. Szajnman for the invaluable chemistry technical assistance and Lic. J. Vrdoljak for his assistance in DT measurements. This work was supported by Argentinian grants from Universidad de Buenos Aires (UBACyT 20020130100058BA), CONICET (PIP 11220150100029CO) and ANPCyT (PICT 2795/2010 and 1121/2013), by Spanish MINECO grants (PIB2010AR-00266 and BIO2012-40244) and cofunded with European Regional Development Funds and Marie Curie IRSES Project DEANN (PIRSES-GA-2013-612583).
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Type:
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Artículo
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