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Rabbit morula vitrification reduces early foetal growth and increases losses throughout gestation

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Rabbit morula vitrification reduces early foetal growth and increases losses throughout gestation

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dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Saenz de Juano Ribes, María de los Desamparados es_ES
dc.contributor.author Jiménez Trigos, María Estrella es_ES
dc.contributor.author Viudes De Castro, María Pilar es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.date.accessioned 2017-05-03T11:39:08Z
dc.date.available 2017-05-03T11:39:08Z
dc.date.issued 2013-12
dc.identifier.issn 0011-2240
dc.identifier.uri http://hdl.handle.net/10251/80418
dc.description.abstract [EN] Several studies have extensively examined structural and biochemical damage induced by cryopreservation that may lead to loss of rabbit embryo viability, but very little information is available on alterations in growth during gestation and at gene expression level. We started our work by comparing the distribution of losses of embryo and foetal development between control and vitrified rabbit morulae. Furthermore, data on foetal sack, foetal and maternal placenta and foetus size for 10-14days of gestation were evaluated by ultrasonography. We reported that vitrification procedure causes detrimental effects on rabbit embryo and foetal development, with two major peaks of losses: one before the implantation (at day 6) and the other during the second part of gestation (after day 14). However, foetal loss may occur during the implantation process and placenta development, as there was a reduction in development of foetus produced from vitrified-warmed embryos between day 10 and 14 of gestation. For these reasons, using a recent microarray study performed in frozen-thawed rabbit embryos as a point of reference, we analysed the effects of vitrification procedure on the expression of 10 candidate genes in 6-day-old blastocysts obtained after vitrification and transfer. We observed that the relative expressions of mRNA transcripts from SCGB1A1, EMP1, ANXA3 and EGFLAM genes were significantly altered. This could help explain why a large number (29%) of vitrified embryos were successfully implanted but subsequently failed to develop to term. Further studies in subsequent embryo-foetal developmental stages, such as initiation of placenta formation, together with more sensitive high-throughput tools, should help us understand the deficiencies that hinder foetal development and identify the repairing mechanism employed by embryos to overcome vitrification effects. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Cryobiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit es_ES
dc.subject Vitrification es_ES
dc.subject Embryo survival es_ES
dc.subject Foetal development es_ES
dc.subject Gene expression es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Rabbit morula vitrification reduces early foetal growth and increases losses throughout gestation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.cryobiol.2013.09.165
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Vicente Antón, JS.; Saenz De Juano Ribes, MDLD.; Jiménez Trigos, ME.; Viudes De Castro, MP.; Peñaranda, D.; Marco Jiménez, F. (2013). Rabbit morula vitrification reduces early foetal growth and increases losses throughout gestation. Cryobiology. 67(3):321-326. doi:10.1016/j.cryobiol.2013.09.165. Epub 2013 Sep 27 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.cryobiol.2013.09.165 es_ES
dc.description.upvformatpinicio 321 es_ES
dc.description.upvformatpfin 326 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 67 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 253732 es_ES


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