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dc.contributor.author | Vicent González, Isabel Elisa | es_ES |
dc.contributor.author | Navarro Marzal, Alfonso Luis | es_ES |
dc.contributor.author | Mulet Salort, José Miguel | es_ES |
dc.contributor.author | Sharma, Sukesh Chander | es_ES |
dc.contributor.author | Serrano Salom, Ramón | es_ES |
dc.date.accessioned | 2017-05-03T12:35:06Z | |
dc.date.available | 2017-05-03T12:35:06Z | |
dc.date.issued | 2015-05 | |
dc.identifier.issn | 1567-1356 | |
dc.identifier.uri | http://hdl.handle.net/10251/80513 | |
dc.description.abstract | The fermenting ability of Saccharomyces at low temperatures is crucial for the development of alcoholic beverages, but the key factors for the cold tolerance of yeast are not well known. In this report, we present the results of a screening for genes able to confer cold tolerance by overexpression in a laboratory yeast strain auxotrophic for tryptophan. We identified genes of tryptophan permeases (TAT1 and TAT2), suggesting that the first limiting factor in the growth of tryptophan auxotrophic yeast at low temperatures is tryptophan uptake. This fact is of little relevance to industrial strains which are prototrophic for tryptophan. Then, we screened for genes able to confer growth at low temperatures in tryptophan-rich media and found several genes related to phosphate uptake (PHO84, PHO87, PHO90 and GTR1). This suggests that without tryptophan limitation, uptake of inorganic phosphate becomes the limiting factor. We have found that overexpression of the previously uncharacterized ORF YCR015c/CTO1 increases the uptake of inorganic phosphate. Also, genes involved in ergosterol biosynthesis (NSG2) cause improvement of growth at 10°C, dependent on tryptophan uptake, while the gluconeogenesis gene PCK1 and the proline biosynthesis gene PRO2 cause an improvement in growth at 10°C, independent of tryptophan and phosphate uptake. | es_ES |
dc.description.sponsorship | I. Vicent was a recipient of a FPI fellowship from the Generalitat Valenciana. This work was supported by Grant AGL2003-03757 from the Spanish Ministry of Science and Technology and by Grant ACOMP06/66 from Generalitat Valenciana (both awarded to A.N.), and funded by Universidad Politecnica de Valencia (Grants PPI2742/2002 and PPI5621-05-04) awarded to A.N. and R.S. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Oxford University Press (OUP): Policy B - Oxford Open Option D | es_ES |
dc.relation.ispartof | FEMS Yeast Research | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Cold stress | es_ES |
dc.subject | Phosphate transport | es_ES |
dc.subject | CTO1 | es_ES |
dc.subject | Tryptophan uptake | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | Uptake of inorganic phosphate is a limiting factor for Saccharomyces cerevisiae during growth at low temperatures | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1093/femsyr/fov008 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//AGL2003-03757/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACOMP06%2F66/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PPI2742%2F2002/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PPI5621-05-04/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Ingeniería de Alimentos para el Desarrollo - Institut Universitari d'Enginyeria d'Aliments per al Desenvolupament | es_ES |
dc.description.bibliographicCitation | Vicent González, IE.; Navarro Marzal, AL.; Mulet Salort, JM.; Sharma, SC.; Serrano Salom, R. (2015). Uptake of inorganic phosphate is a limiting factor for Saccharomyces cerevisiae during growth at low temperatures. FEMS Yeast Research. 15(3):1-13. https://doi.org/10.1093/femsyr/fov008 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/femsyr/fov008 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 13 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 15 | es_ES |
dc.description.issue | 3 | es_ES |
dc.relation.senia | 284831 | es_ES |
dc.contributor.funder | Ministerio de Ciencia y Tecnología | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |