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Peptidyl-prolyl cis-trans isomerase ROF2 modulates intracellular pH homeostasis in Arabidopsis

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Peptidyl-prolyl cis-trans isomerase ROF2 modulates intracellular pH homeostasis in Arabidopsis

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dc.contributor.author Bissoli, Gaetano es_ES
dc.contributor.author Niñoles Rodenes, Regina es_ES
dc.contributor.author Fresquet Corrales, Sandra es_ES
dc.contributor.author Palombieri, Samuela es_ES
dc.contributor.author Bueso Ródenas, Eduardo es_ES
dc.contributor.author Rubio, Lourdes es_ES
dc.contributor.author Garcia-Sanchez, Maria J. es_ES
dc.contributor.author Fernandez, Jose A. es_ES
dc.contributor.author Mulet Salort, José Miguel es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.date.accessioned 2017-05-04T10:54:11Z
dc.date.available 2017-05-04T10:54:11Z
dc.date.issued 2012-05
dc.identifier.issn 0960-7412
dc.identifier.uri http://hdl.handle.net/10251/80571
dc.description.abstract [EN] Intracellular pH must be kept close to neutrality to be compatible with cellular functions, but the mechanisms of pH homeostasis and the responses to intracellular acidification are mostly unknown. In the plant Arabidopsis thaliana, we found that intracellular acid stress generated by weak organic acids at normal external pH induces expression of several chaperone genes, including ROF2, which encodes a peptidyl-prolyl cis-trans isomerase of the FK506-binding protein class. Loss of function of ROF2, and especially double mutation of ROF2 and the closely related gene ROF1, results in acid sensitivity. Over-expression of ROF2 confers tolerance to intracellular acidification by increasing proton extrusion from cells. The activation of the plasma membrane proton pump (H+-ATPase) is indirect: over-expression of ROF2 activates K+ uptake, causing depolarization of the plasma membrane, which activates the electrogenic H+ pump. The depolarization of ROF2 over-expressing plants explains their tolerance to toxic cations such as lithium, norspermidine and hygromycin B, whose uptake is driven by the membrane potential. As ROF2 induction and intracellular acidification are common consequences of many stresses, this mechanism of pH homeostasis may be of general importance for stress tolerance. es_ES
dc.description.sponsorship This work was supported by grants BFU2008-00604 from the Ministerio de Ciencia e Innovacion (Madrid, Spain) and PROMETEO/2010/ 038 of the 'Conselleria de Educacion' (Valencia, Spain). We thank Dr Eugenio Grau (Sequencing Service, Instituto de Biologia Molecular y Celular de Plantas, Valencia, Spain) for sequencing of the various genes, and Dr Vicente Fornes (Instituto de Tecnologia Quimica, Valencia, Spain) for assistance with atomic absorption spectrophotometry. None of the authors has a conflict of interest to declare.
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//BFU2008-00604/ES/REGULACION DE H+-ATPASA, TRANSPORTE DE K+ Y TOR: INVESTIGANDO LA RELACION ENTRE DETERMINANTES BIOFISICOS Y BIOQUIMICOS DEL CRECIMIENTO CELULAR EN ARABIDOPSIS/ es_ES
dc.relation GV/PROMETEO/2010/ 038 es_ES
dc.relation.ispartof Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject H plus-ATPase es_ES
dc.subject K plus transport es_ES
dc.subject Membrane potential es_ES
dc.subject Toxic cations es_ES
dc.subject Stress tolerance es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Peptidyl-prolyl cis-trans isomerase ROF2 modulates intracellular pH homeostasis in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1365-313X.2012.04921.x.
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Bissoli, G.; Niñoles Rodenes, R.; Fresquet Corrales, S.; Palombieri, S.; Bueso Ródenas, E.; Rubio, L.; Garcia-Sanchez, MJ.... (2012). Peptidyl-prolyl cis-trans isomerase ROF2 modulates intracellular pH homeostasis in Arabidopsis. Plant Journal. 70(4):704-716. https://doi.org/10.1111/j.1365-313X.2012.04921.x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1111/j.1365-313X.2012.04921.x es_ES
dc.description.upvformatpinicio 704 es_ES
dc.description.upvformatpfin 716 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 70 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 223012 es_ES
dc.identifier.pmid 22268595
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Generalitat Valenciana


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