Vilchez Olivencia, MC.; Morini, M.; Peñaranda, D.; Gallego Albiach, V.; Asturiano Nemesio, JF.; Pérez Igualada, LM. (2016). Sodium affects the sperm motility in the European eel. Comparative Biochemistry and Physiology - B: Comparative Biochemistry. 198:51-58. doi:10.1016/j.cbpa.2016.04.008
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/80574
Title:
|
Sodium affects the sperm motility in the European eel
|
Author:
|
Vilchez Olivencia, Maria Carmen
MORINI, MARINA
Peñaranda, D.S.
Gallego Albiach, Victor
Asturiano Nemesio, Juan Francisco
Pérez Igualada, Luz María
|
UPV Unit:
|
Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal
Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
|
Issued date:
|
|
Abstract:
|
[EN] The role of seminal plasma sodium and activation media sodium on sperm motility was examined by selectively removing the element from these two media, in European eel sperm. Sperm size (sperm head area) was also ...[+]
[EN] The role of seminal plasma sodium and activation media sodium on sperm motility was examined by selectively removing the element from these two media, in European eel sperm. Sperm size (sperm head area) was also measured using an ASMA (Automated Sperm Morphometry Analyses) system, in the different conditions. Intracellular sodium [Na+](i) was quantitatively analyzed by first time in the spermatozoa from a marine fish species. Measurement of [Na+](i) was done before and after motility activation, by Flow Cytometry, using CoroNa Green AM as a dye. Sperm motility activation induced an increase in [Na+](i) from 96.72 mM in quiescent stage to 152.21 mM post-activation in seawater. A significant decrease in sperm head area was observed post activation in seawater. There was a notable reduction in sperm motility when sodium was removed from the seminal plasma, but not when it was removed from the activation media. Sodium removal was also linked to a significant reduction in sperm head area in comparison to the controls. Our results indicate that the presence of the ion Na+ in the seminal plasma (or in the extender medium) is necessary for the preservation of sperm motility in European eel, probably because it plays a role in maintaining an appropriate sperm cell volume in the quiescent stage of the spermatozoa. (C) 2016 Elsevier Inc All rights reserved.
[-]
|
Subjects:
|
Anguilla anguilla
,
Ion channels
,
Flow cytometry
,
Calibration
,
CASA
,
ASMA
,
Cell volume
,
Monensin
,
Amiloride
,
Intracellular sodium
|
Copyrigths:
|
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
|
Source:
|
Comparative Biochemistry and Physiology - B: Comparative Biochemistry. (issn:
0305-0491
) (eissn:
1531-4332
)
|
DOI:
|
10.1016/j.cbpa.2016.04.008
|
Publisher:
|
Elsevier
|
Publisher version:
|
http://dx.doi.org/10.1016/j.cbpa.2016.04.008
|
Project ID:
|
SPERMOT project (Spanish Ministry of Science and Innovation, MICINN) AGL2010-16009
...[+]
SPERMOT project (Spanish Ministry of Science and Innovation, MICINN) AGL2010-16009
UPV PAID Program 2011-S2-02-6521
Generalitat Valenciana (Programa Grisolia) GRISOLIA/2012/006
UPV PAID-10-14 PTA2011-4948-I
MICINN PTA2011-4948-I
COST Office FA1205
[-]
|
Thanks:
|
Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Program (2011-S2-02-6521), Marina Morini has a predoctoral grant from ...[+]
Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Program (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN (PTA2011-4948-I) and UPV (PTA2011-4948-I). Grants to attend meetings were received from COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE).
[-]
|
Type:
|
Artículo
|