Romero Rueda, T.; Beltrán Martínez, MC.; Lisandro Althaus, R.; Molina Pons, MP. (2014). Detection of antibiotics in goat's milk; effect of detergents on the response of microbial inhibitor tests. Journal of Dairy Research. 81(3):372-377. https://doi.org/10.1017/S0022029914000259
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/80584
Title:
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Detection of antibiotics in goat's milk; effect of detergents on the response of microbial inhibitor tests
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Author:
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Romero Rueda, Tamara
Beltrán Martínez, Mª Carmen
Lisandro Althaus, Rafael
Molina Pons, Mª Pilar
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UPV Unit:
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Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal
Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
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Issued date:
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Abstract:
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[EN] The aim of the study was to evaluate the interference of acid and alkaline detergents employed in the cleaning of milking equipment of caprine dairy farms on the performance of microbial tests used in antibiotic control ...[+]
[EN] The aim of the study was to evaluate the interference of acid and alkaline detergents employed in the cleaning of milking equipment of caprine dairy farms on the performance of microbial tests used in antibiotic control (BRT MRL, Delvotest MCS, and Eclipse 100). Eight concentrations of commercial detergents, five acid (0-0.25%) and five alkaline (0-1%) were add to antimicrobial-free goat's milk to evaluate the detergent effect on the response of microbial inhibitor tests. To evaluate the effect of detergents on the detection capability of microbial tests two detergents at 0.5ml/l (one acid and one basic) and eight concentrations of four beta-lactam antibiotics (ampicillin, amoxicillin, cloxacillin and benzylpenicillin) were used. Milk without detergents was used as control. The spiked samples were analysed twelve times by three microbial tests. The results showed that the presence of acid detergents did not affect the response of microbial tests for any of the concentrations tested. However, at concentrations equal to or greater than 2ml/l alkaline detergents positive results were found in microbial tests (16.7-100%). The detection limits of the screening tests for penicillins were not modified substantially by the presence of detergents. In general, the presence of acid and alkaline detergents in goat's milk did not produce a great interference in the microbial tests, only high concentrations of detergents could cause non-compliant results, but these concentrations are difficult to find in practice if proper cleaning procedures are applied in goat dairy farms.
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Subjects:
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Detergents
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Inhibitors
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Screening methods
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Goat's milk
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Journal of Dairy Research. (issn:
0022-0299
) (eissn:
1469-7629
)
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DOI:
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10.1017/S0022029914000259
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Publisher:
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Cambridge University Press (CUP)
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Publisher version:
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http://doi.org/10.1017/S0022029914000259
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Project ID:
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info:eu-repo/grantAgreement/MICINN//AGL2009-11524/ES/Estrategia Analitica Para La Deteccion De Residuos De Antibioticos En La Leche De Oveja Y Cabra/
info:eu-repo/grantAgreement/GVA//ACOMP%2F2012%2F164/
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Thanks:
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This work forms part of the Project AGL 2009-11524 financed by the Ministry of Science and Innovation (Madrid, Spain) and the Generalitat Valenciana ACOMOP/2012/164 (Valencia, Spain). The authors are grateful to AiM Analytik ...[+]
This work forms part of the Project AGL 2009-11524 financed by the Ministry of Science and Innovation (Madrid, Spain) and the Generalitat Valenciana ACOMOP/2012/164 (Valencia, Spain). The authors are grateful to AiM Analytik in MilchProduktions-und Vertriebs-GmbH (Munich, Germany), DSM Food Specialties (Delft, Netherlands), ZEU-Inmunotec (Zaragoza, Spain) for their technological support.
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Type:
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Artículo
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