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Novel microbiological system for antibiotic detection in ovine milk

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Novel microbiological system for antibiotic detection in ovine milk

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dc.contributor.author Nagel, Orlando Guillermo es_ES
dc.contributor.author Beltrán Martínez, Mª Carmen es_ES
dc.contributor.author Molina Pons, Mª Pilar es_ES
dc.contributor.author Lisandro Althaus, Rafael es_ES
dc.date.accessioned 2017-05-04T12:33:55Z
dc.date.available 2017-05-04T12:33:55Z
dc.date.issued 2012-01-01 es_ES
dc.identifier.issn 0921-4488 es_ES
dc.identifier.uri http://hdl.handle.net/10251/80586
dc.description.abstract [EN] This article presents a microbiological system composed of a " BT" bioassay (Beta-lactams and Tetracyclines) and a " QS" bioassay (Quinolones and Sulfonamides). The " BT" bioassay contains spores of Geobacillus stearothermophilus, bromocresol purple and cloramphenicol in a culture medium (incubation time: 2.45h), while the " QS" bioassay uses spores of Bacillus subtilis, trifenyltetrazolium - toluidine blue and trimethoprim in a suitable culture medium (incubation time: 5.5h). The detection capability (CC ß) of 27 antimicrobial agents in ovine milk were determined by logistic regression models. Thus, the " BT" bioassay detects amoxycillin, ampicillin, penicillin " G" , cloxacillin, oxacillin, cephalexin, cefoperazone, ceftiofur, chlortetracycline, oxytetracycline, tetracycline, neomycin, gentamycin and tylosin, while " QS" bioassay detects: ciprofloxacin, enrofloxacin, marbofloxacin, sulfadiazine, sulfadimethoxine, sulfamerazine, sulfamethazine, sulfamethoxazole, sulfathiazole, erythromycin, lincomycin and spiramycin at levels close to their respective Maximum Residue Limits. The simultaneous use of both bioassays detects a large number of antibiotics in milk given each method's adequate complementary sensitivity. © 2011 Elsevier B.V.. es_ES
dc.description.sponsorship This research work has been carried out as part of the CAI+D'09/12 Projects (No. 033-173 Res. H.C.D. No. 100/09, Universidad Nacional del Litoral, Santa Fe, Argentina) and AGL2009-11524 (Ministry of Science and Innovation, Spain). en_EN
dc.language Inglés
dc.publisher Elsevier es_ES
dc.relation Universidad Nacional del Litoral/033-173100/09 es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//AGL2009-11524/ES/Estrategia Analitica Para La Deteccion De Residuos De Antibioticos En La Leche De Oveja Y Cabra/ es_ES
dc.relation.ispartof Small Ruminant Research es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Bioassay es_ES
dc.subject Detection es_ES
dc.subject Ovine milk es_ES
dc.subject System es_ES
dc.subject Bacillus subtilis es_ES
dc.subject Bacteria (microorganisms) es_ES
dc.subject Geobacillus stearothermophilus es_ES
dc.subject Ovis es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Novel microbiological system for antibiotic detection in ovine milk
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.smallrumres.2011.11.018 es_ES
dc.rights.accessRights Abierto
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Nagel, OG.; Beltrán Martínez, MC.; Molina Pons, MP.; Lisandro Althaus, R. (2012). Novel microbiological system for antibiotic detection in ovine milk. Small Ruminant Research. 102(1):26-31. https://doi.org/10.1016/j.smallrumres.2011.11.018 es_ES
dc.description.accrualMethod S es_ES
dc.description.upvformatpinicio 26 es_ES
dc.description.upvformatpfin 31 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 102 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 209226 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Universidad Nacional del Litoral, Argentina


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