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Visual Tracking of Plant Virus Infection and Movement Using a Reporter MYB Transcription Factor That Activates Anthocyanin Biosynthesis

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Visual Tracking of Plant Virus Infection and Movement Using a Reporter MYB Transcription Factor That Activates Anthocyanin Biosynthesis

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dc.contributor.author Bedoya, Leonor C. es_ES
dc.contributor.author Martinez Garcia, Fernando es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2017-05-04T12:40:51Z
dc.date.available 2017-05-04T12:40:51Z
dc.date.issued 2012-03
dc.identifier.issn 0032-0889
dc.identifier.uri http://hdl.handle.net/10251/80588
dc.description.abstract Insertion of reporter genes into plant virus genomes is a common experimental strategy to research many aspects of the viral infection dynamics. Their numerous advantages make fluorescent proteins the markers of choice in most studies. However, the use of fluorescent proteins still has some limitations, such as the need of specialized material and facilities to detect the fluorescence. Here, we demonstrate a visual reporter marker system to track virus infection and movement through the plant. The reporter system is based on expression of Antirrhinum majus MYB-related Rosea1 (Ros1) transcription factor (220 amino acids; 25.7 kD) that activates a series of biosynthetic genes leading to accumulation of colored anthocyanins. Using two different tobacco etch potyvirus recombinant clones tagged with Ros1, we show that infected tobacco (Nicotiana tabacum) tissues turn bright red, demonstrating that in this context, the sole expression of Ros1 is sufficient to induce pigment accumulation to a level readily detectable to the naked eye. This marker system also reports viral load qualitatively and quantitatively by means of a very simple extraction process. The Ros1 marker remained stable within the potyvirus genome through successive infectious passages from plant to plant. The main limitation of this marker system is that color output will depend on each particular plant host-virus combination and must be previously tested. However, our experiments demonstrate accurate tracking of turnip mosaic potyvirus infecting Arabidopsis (Arabidopsis thaliana) and either tobacco mosaic virus or potato X virus infecting Nicotiana benthamiana, stressing the general applicability of the method. es_ES
dc.description.sponsorship This work was supported by the Ministerio de Ciencia e Innovacion (Spain; grant nos. BIO2008-01986 and BIO2011-26741 and also partially by grant no. BIO2010-15384) and by the Universidad Politecnica de Valencia (Spain; predoctoral fellowship to F.M.). en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation Ministerio de Ciencia e Innovacion (Spain) BIO2008-01986 es_ES
dc.relation Ministerio de Ciencia e Innovacion (Spain) BIO2011-26741 es_ES
dc.relation Ministerio de Ciencia e Innovacion (Spain) BIO2010-15384 es_ES
dc.relation Universidad Politecnica de Valencia (Spain) es_ES
dc.relation.ispartof Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Visual Tracking of Plant Virus Infection and Movement Using a Reporter MYB Transcription Factor That Activates Anthocyanin Biosynthesis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1104/pp.111.192922
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Bedoya, LC.; Martínez García, F.; Orzáez Calatayud, DV.; Daros Arnau, JA. (2012). Visual Tracking of Plant Virus Infection and Movement Using a Reporter MYB Transcription Factor That Activates Anthocyanin Biosynthesis. Plant Physiology. 158(3):1130-1138. doi:10.1104/pp.111.192922 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1104/pp.111.192922 es_ES
dc.description.upvformatpinicio 1130 es_ES
dc.description.upvformatpfin 1138 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 158 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 233306 es_ES


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