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Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase

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Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase

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dc.contributor.author Krzywinska, Ewa es_ES
dc.contributor.author Bucholc, Maria es_ES
dc.contributor.author Kulik, Anna es_ES
dc.contributor.author Ciesielski, Arkadiusz es_ES
dc.contributor.author Lichocka, Malgorzata es_ES
dc.contributor.author Debski, Janusz es_ES
dc.contributor.author Ludwikow, Agnieszka es_ES
dc.contributor.author Dadlez, Michal es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.contributor.author Dobrowolska, Grazyna es_ES
dc.date.accessioned 2017-05-09T11:48:35Z
dc.date.available 2017-05-09T11:48:35Z
dc.date.issued 2016-06-13
dc.identifier.issn 1471-2229
dc.identifier.uri http://hdl.handle.net/10251/80769
dc.description.abstract Background: SNF1-related protein kinases 2 (SnRK2s) are key regulators of the plant response to osmotic stress. They are transiently activated in response to drought and salinity. Based on a phylogenetic analysis SnRK2s are divided into three groups. The classification correlates with their response to abscisic acid (ABA); group 1 consists SnRK2s non-activated in response to ABA, group 2, kinases non-activated or weakly activated (depending on the plant species) by ABA treatment, and group 3, ABA-activated kinases. The activity of all SnRK2s is regulated by phosphorylation. It is well established that clade A phosphoprotein phosphatases 2C (PP2Cs) are negative regulators of ABA-activated SnRK2s, whereas regulators of SnRK2s from group 1 remain unidentified. Results: Here, we show that ABI1, a PP2C clade A phosphatase, interacts with SnRK2.4, member of group 1 of the SnRK2 family, dephosphorylates Ser158, whose phosphorylation is needed for the kinase activity, and inhibits the kinase, both in vitro and in vivo. Our data indicate that ABI1 and the kinase regulate primary root growth in response to salinity; the phenotype of ABI1 knockout mutant (abi1td) exposed to salt stress is opposite to that of the snrk2.4 mutant. Moreover, we show that the activity of SnRK2s from group 1 is additionally regulated by okadaic acid-sensitive phosphatase(s) from the phosphoprotein phosphatase (PPP) family. Conclusions: Phosphatase ABI1 and okadaic acid-sensitive phosphatases of the PPP family are negative regulators of salt stress-activated SnRK2.4. The results show that ABI1 inhibits not only the ABA-activated SnRK2s but also at least one ABA-non-activated SnRK2, suggesting that the phosphatase is involved in the cross talk between ABA-dependent and ABA-independent stress signaling pathways in plants. es_ES
dc.description.sponsorship This work was supported by the Ministry of Science and Higher Education, Poland (grant 500/N-COST/2009/0 to GD) and National Science Centre (grant 2011/03/B/NZ3/00297 to GD). Funding in the laboratory of Pedro L. Rodriguez was provided by grant BIO2014-52537-R. Financial support from the EU (COST FA0605) is gratefully acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Salinity es_ES
dc.subject Osmotic stress signaling es_ES
dc.subject SNF1-related protein kinases 2 es_ES
dc.subject SnRK2 es_ES
dc.subject Phosphoprotein phosphatases es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject ABI1 es_ES
dc.subject PPP es_ES
dc.subject PP2C es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-016-0817-1
dc.relation.projectID info:eu-repo/grantAgreement/MNiSW//500%2FN-COST%2F2009%2F0/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COST//FA0605/EU/Signaling control of stress tolerance and production of stress protective compounds in plants/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NCN//2011%2F03%2FB%2FNZ3%2F00297/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2014-52537-R/ES/REGULACION DE LA SEÑALIZACION DEL ABA MEDIANTE MECHANISMOS QUE AFECTAN LOCALIZACION SUBCELULAR, VIDA MEDIA Y ACTIVIDAD DE RECEPTORES PARA REFORZAR TOLERANCIA VEGETAL A SEQUIA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Krzywinska, E.; Bucholc, M.; Kulik, A.; Ciesielski, A.; Lichocka, M.; Debski, J.; Ludwikow, A.... (2016). Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase. BMC Plant Biology. 16(136):1-12. https://doi.org/10.1186/s12870-016-0817-1 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1186/s12870-016-0817-1 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 136 es_ES
dc.relation.senia 332385 es_ES
dc.identifier.pmid 27297076 en_EN
dc.identifier.pmcid PMC4907068 en_EN
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder European Cooperation in Science and Technology es_ES
dc.contributor.funder National Science Centre, Polonia es_ES
dc.contributor.funder Ministry of Science and Higher Education, Polonia es_ES
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