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Effect of Embryonic Genotype on Reference Gene Selection for RT-qPCR Normalization

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Effect of Embryonic Genotype on Reference Gene Selection for RT-qPCR Normalization

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Llobat, L.; Marco Jiménez, F.; Peñaranda, D.; Saenz De Juano Ribes, MDLD.; Vicente Antón, JS. (2012). Effect of Embryonic Genotype on Reference Gene Selection for RT-qPCR Normalization. Reproduction in Domestic Animals. 47(4):629-634. doi:10.1111/j.1439-0531.2011.01934.x

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/80810

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Title: Effect of Embryonic Genotype on Reference Gene Selection for RT-qPCR Normalization
Author:
UPV Unit: Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal
Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
Issued date:
Abstract:
[EN] Contents To obtain more reliable results with biological significance, it requires data normalization using an appropriate internal control gene. In rabbits, there are classic stable reference genes that have been ...[+]
Subjects: Bovine preimplantation embryos , Transcription PCR data , Time quantitative PCR , Real time , Housekeeping genes , In vitro , Relative quantification , Expression , Validation , Oocytes
Copyrigths: Cerrado
Source:
Reproduction in Domestic Animals. (issn: 0936-6768 )
DOI: 10.1111/j.1439-0531.2011.01934.x
Publisher:
Wiley
Publisher version: http://doi.org/10.1111/j.1439-0531.2011.01934.x
Thanks:
We are grateful to the Researcher Personal Training Programme from Valencia Polytechnic University. This work was supported by the Spanish Research Project (CICYT AGL2008-03274) and Generalitat Valenciana Research Programme ...[+]
Type: Artículo

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