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Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid

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Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid

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dc.contributor.author González Guzmán, Miguel es_ES
dc.contributor.author Pizzio Bianchi, Gaston Alfredo es_ES
dc.contributor.author Antoni-Alandes, Regina es_ES
dc.contributor.author Vera Sirera, Francisco José es_ES
dc.contributor.author Merilo, Ebe es_ES
dc.contributor.author Bassel, George es_ES
dc.contributor.author Fernandez, M.A. es_ES
dc.contributor.author Holdsworth, M.J. es_ES
dc.contributor.author Perez Amador, Miguel Angel es_ES
dc.contributor.author kollist, H es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.date.accessioned 2017-05-12T12:17:06Z
dc.date.available 2017-05-12T12:17:06Z
dc.date.issued 2012-06
dc.identifier.issn 1040-4651
dc.identifier.uri http://hdl.handle.net/10251/81086
dc.description.abstract [EN] Abscisic acid (ABA) is a key hormone for plant growth, development, and stress adaptation. Perception of ABA through four types of receptors has been reported. We show here that impairment of ABA perception through the PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) branch reduces vegetative growth and seed production and leads to a severe open stomata and ABA-insensitive phenotype, even though other branches for ABA perception remain functional. An Arabidopsis thaliana sextuple mutant impaired in six PYR/PYL receptors, namely PYR1, PYL1, PYL2, PYL4, PYL5, and PYL8, was able to germinate and grow even on 100 mu M ABA. Whole-rosette stomatal conductance (Gst) measurements revealed that leaf transpiration in the sextuple pyr/pyl mutant was higher than in the ABA-deficient aba3-1 or ABA-insensitive snrk2.6 mutants. The gradually increasing Gst values of plants lacking three, four, five, and six PYR/PYLs indicate quantitative regulation of stomatal aperture by this family of receptors. The sextuple mutant lacked ABA-mediated activation of SnRK2s, and ABA-responsive gene expression was dramatically impaired as was reported in snrk2.2/2.3/2.6. In summary, these results show that ABA perception by PYR/PYLs plays a major role in regulation of seed germination and establishment, basal ABA signaling required for vegetative and reproductive growth, stomatal aperture, and transcriptional response to the hormone. es_ES
dc.description.sponsorship We thank Joseph Ecker and the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants, the Arabidopsis Biological Resource Center/Nottingham Arabidopsis Stock Centre for distributing these seeds, and Sean Cutler for the pyr1-1, 114 and 1124 mutants. This study was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (grant BIO2011-23446 to P. L. R; fellowship to R. A.; Juan de la Cierva contract to M. G.-G.), by the Estonian Ministry of Science and Education (ETF7763 and ETF9208, SF0180071s07), by the European Regional Fund (Center of Excellence in Environmental Adaptation), and by a Marie Curie Incoming International Fellowship to G. W. B. en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation Ministerio de Ciencia e Innovación, Spain es_ES
dc.relation Estonian Ministry of Science and Education (ETF7763 and ETF9208, SF0180071s07) es_ES
dc.relation.ispartof Plant Cell es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject CHELATASE H-SUBUNIT es_ES
dc.subject Activated protein-kinases es_ES
dc.subject PHOSPHATASES TYPE 2C es_ES
dc.subject Gene-expression es_ES
dc.subject Molecular-basis es_ES
dc.subject Root-growth es_ES
dc.subject ABA es_ES
dc.subject Stress es_ES
dc.subject Identification es_ES
dc.subject Inhibition es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1105/tpc.112.098574
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Gonzalez Guzman, M.; Pizzio Bianchi, GA.; Antoni-Alandes, R.; Vera Sirera, FJ.; Merilo, E.; Bassel, G.; Fernandez, M.... (2012). Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid. Plant Cell. 24(6):2483-2496. doi:10.1105/tpc.112.098574 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1105/tpc.112.098574 es_ES
dc.description.upvformatpinicio 2483 es_ES
dc.description.upvformatpfin 2496 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 24 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 232609 es_ES
dc.identifier.eissn 1532-298X
dc.identifier.pmid 30482863 es_ES
dc.identifier.pmcid PMC3406898


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