dc.contributor.author |
Escobar-Bravo, Rocio
|
es_ES |
dc.contributor.author |
Alba, Juan M.
|
es_ES |
dc.contributor.author |
Pons Puig, Clara
|
es_ES |
dc.contributor.author |
Granell Richart, Antonio
|
es_ES |
dc.contributor.author |
Kant, Merijn R.
|
es_ES |
dc.contributor.author |
Moriones, Enrique
|
es_ES |
dc.contributor.author |
Fernandez-Munoz, Rafael
|
es_ES |
dc.date.accessioned |
2017-05-15T10:08:19Z |
|
dc.date.available |
2017-05-15T10:08:19Z |
|
dc.date.issued |
2016-11-22 |
|
dc.identifier.issn |
1664-462X |
|
dc.identifier.uri |
http://hdl.handle.net/10251/81127 |
|
dc.description.abstract |
Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated tomatoes lack many of the resistances of their wild relatives. In order to increase protection to its major pest, the whitefly Bemisia tabaci and its transmitted Tomato Yellow Leaf Curl Virus (TYLCV), we introgressed a trichome-based resistance trait from the wild tomato Solanum pimpinellifolium into cultivated tomato, Solanum lycopersicum. The tomato backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike cultivated tomatoes, and exhibits increased, yet limited protection to whiteflies at early development stages. Treatment of young plants with methyl jasmonate (MeJA) resulted in a 60% increase in type-IV trichome density, acylsucrose production, and enhanced resistance to whiteflies, leading to 50% decrease in the virus disease incidence compared to cultivated tomato. Using transcriptomics, metabolite analysis, and insect bioassays we established the basis of this inducible resistance. We found that MeJA activated the expression of the genes involved in the biosynthesis of the defensive acylsugars in young BC5S2 plants leading to enhanced chemical defenses in their acquired type-IV trichomes. Our results show that not only constitutive but also these inducible defenses can be transferred from wild into cultivated crops to aid sustainable protection, suggesting that conventional breeding strategies provide a feasible alternative to increase pest resistance in tomato |
es_ES |
dc.description.sponsorship |
This work was funded by grants AGL2013-48913-C2-01-R/AGR (Spanish MICINN, co-financed by EU FEDER) and P10-AGR6516 (Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain). RE-B was recipient of a FPI grant by MICINN JA was supported by the Netherlands Organization for Scientific Research NWO (Earth and Life Sciences ALW/TOP 854.11.005). MK was supported by Netherlands Organization for Scientific Research NWO (Technology Foundation STW/VIDI 13492). EM and RF-M are members of AGR-214 and AGR-129 research groups, respectively, partially funded by the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain (cofinanced by EU FEDER-FSE). AG was funded by TRADITOM (634561) under the H2020 SFS-07a-2014 EU Program, the Spanish Ministry BIO2013-42193-R, and the EU COST-FA1106. |
|
dc.language |
Inglés |
es_ES |
dc.publisher |
Frontiers Media |
es_ES |
dc.relation.ispartof |
Frontiers in Plant Science |
es_ES |
dc.rights |
Reconocimiento (by) |
es_ES |
dc.subject |
Acylsugars |
es_ES |
dc.subject |
Bemisia tabaci |
es_ES |
dc.subject |
Plant breeding |
es_ES |
dc.subject |
Methyl jasmonate |
es_ES |
dc.subject |
Tomato |
es_ES |
dc.subject |
Tomato yellow leaf curl virus |
es_ES |
dc.subject |
Trichomes |
es_ES |
dc.subject |
Whitefly |
es_ES |
dc.title |
A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence |
es_ES |
dc.type |
Artículo |
es_ES |
dc.identifier.doi |
10.3389/fpls.2016.01732 |
|
dc.relation.projectID |
info:eu-repo/grantAgreement/MINECO//AGL2013-48913-C2-1-R/ES/EMERGENCIA VIRAL Y MOSCA BLANCA: INTERACCIONES MULTITROFICAS EN EL PATOSISTEMA GEMINIVIRUS%2FCRINIVIRUS-BEMISIA TABACI-TOMATE/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/EC/H2020/634561/EU/Traditional tomato varieties and cultural practices: a case for agricultural diversification with impact on food security and health of European population/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/NWO//STW%2FVIDI 13492/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/NWO//854.11.005/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/COST//FA1106/EU/An integrated systems approach to determine the developmental mechanisms controlling fleshy fruit quality in tomato and grapevine/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/Junta de Andalucía//P10-AGR-6516/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/MINECO//BIO2013-42193-R/ES/GREEN SWITCHES: DISEÑO DE CIRCUITOS GENETICOS ARTIFICIALES PARA LA PRODUCCION DE PROTEINAS RECOMBINANTES Y EL ENRIQUECIMIENTO NUTRICIONAL DE PLANTAS SOLANACEAS/ |
es_ES |
dc.rights.accessRights |
Abierto |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes |
es_ES |
dc.description.bibliographicCitation |
Escobar-Bravo, R.; Alba, JM.; Pons Puig, C.; Granell Richart, A.; Kant, MR.; Moriones, E.; Fernandez-Munoz, R. (2016). A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence. Frontiers in Plant Science. 7(1732):1-16. https://doi.org/10.3389/fpls.2016.01732 |
es_ES |
dc.description.accrualMethod |
S |
es_ES |
dc.relation.publisherversion |
http://dx.doi.org/10.3389/fpls.2016.01732 |
es_ES |
dc.description.upvformatpinicio |
1 |
es_ES |
dc.description.upvformatpfin |
16 |
es_ES |
dc.type.version |
info:eu-repo/semantics/publishedVersion |
es_ES |
dc.description.volume |
7 |
es_ES |
dc.description.issue |
1732 |
es_ES |
dc.relation.senia |
331619 |
es_ES |
dc.identifier.pmid |
27920785 |
en_EN |
dc.identifier.pmcid |
PMC5118631 |
en_EN |
dc.contributor.funder |
European Commission |
|
dc.contributor.funder |
European Cooperation in Science and Technology |
es_ES |
dc.contributor.funder |
Netherlands Organization for Scientific Research |
|
dc.contributor.funder |
Junta de Andalucía |
|
dc.contributor.funder |
Ministerio de Economía y Competitividad |
|
dc.contributor.funder |
European Regional Development Fund |
es_ES |