- -

A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

  • Estadisticas de Uso

A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence

Show simple item record

Files in this item

dc.contributor.author Escobar-Bravo, Rocio es_ES
dc.contributor.author Alba, Juan M. es_ES
dc.contributor.author Pons Puig, Clara es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Kant, Merijn R. es_ES
dc.contributor.author Moriones, Enrique es_ES
dc.contributor.author Fernandez-Munoz, Rafael es_ES
dc.date.accessioned 2017-05-15T10:08:19Z
dc.date.available 2017-05-15T10:08:19Z
dc.date.issued 2016-11-22
dc.identifier.issn 1664-462X
dc.identifier.uri http://hdl.handle.net/10251/81127
dc.description.abstract Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated tomatoes lack many of the resistances of their wild relatives. In order to increase protection to its major pest, the whitefly Bemisia tabaci and its transmitted Tomato Yellow Leaf Curl Virus (TYLCV), we introgressed a trichome-based resistance trait from the wild tomato Solanum pimpinellifolium into cultivated tomato, Solanum lycopersicum. The tomato backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike cultivated tomatoes, and exhibits increased, yet limited protection to whiteflies at early development stages. Treatment of young plants with methyl jasmonate (MeJA) resulted in a 60% increase in type-IV trichome density, acylsucrose production, and enhanced resistance to whiteflies, leading to 50% decrease in the virus disease incidence compared to cultivated tomato. Using transcriptomics, metabolite analysis, and insect bioassays we established the basis of this inducible resistance. We found that MeJA activated the expression of the genes involved in the biosynthesis of the defensive acylsugars in young BC5S2 plants leading to enhanced chemical defenses in their acquired type-IV trichomes. Our results show that not only constitutive but also these inducible defenses can be transferred from wild into cultivated crops to aid sustainable protection, suggesting that conventional breeding strategies provide a feasible alternative to increase pest resistance in tomato es_ES
dc.description.sponsorship This work was funded by grants AGL2013-48913-C2-01-R/AGR (Spanish MICINN, co-financed by EU FEDER) and P10-AGR6516 (Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain). RE-B was recipient of a FPI grant by MICINN JA was supported by the Netherlands Organization for Scientific Research NWO (Earth and Life Sciences ALW/TOP 854.11.005). MK was supported by Netherlands Organization for Scientific Research NWO (Technology Foundation STW/VIDI 13492). EM and RF-M are members of AGR-214 and AGR-129 research groups, respectively, partially funded by the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain (cofinanced by EU FEDER-FSE). AG was funded by TRADITOM (634561) under the H2020 SFS-07a-2014 EU Program, the Spanish Ministry BIO2013-42193-R, and the EU COST-FA1106.
dc.language Inglés es_ES
dc.publisher Frontiers Media es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Acylsugars es_ES
dc.subject Bemisia tabaci es_ES
dc.subject Plant breeding es_ES
dc.subject Methyl jasmonate es_ES
dc.subject Tomato es_ES
dc.subject Tomato yellow leaf curl virus es_ES
dc.subject Trichomes es_ES
dc.subject Whitefly es_ES
dc.title A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2016.01732
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2013-48913-C2-1-R/ES/EMERGENCIA VIRAL Y MOSCA BLANCA: INTERACCIONES MULTITROFICAS EN EL PATOSISTEMA GEMINIVIRUS%2FCRINIVIRUS-BEMISIA TABACI-TOMATE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634561/EU/Traditional tomato varieties and cultural practices: a case for agricultural diversification with impact on food security and health of European population/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NWO//STW%2FVIDI 13492/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NWO//854.11.005/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COST//FA1106/EU/An integrated systems approach to determine the developmental mechanisms controlling fleshy fruit quality in tomato and grapevine/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Andalucía//P10-AGR-6516/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2013-42193-R/ES/GREEN SWITCHES: DISEÑO DE CIRCUITOS GENETICOS ARTIFICIALES PARA LA PRODUCCION DE PROTEINAS RECOMBINANTES Y EL ENRIQUECIMIENTO NUTRICIONAL DE PLANTAS SOLANACEAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Escobar-Bravo, R.; Alba, JM.; Pons Puig, C.; Granell Richart, A.; Kant, MR.; Moriones, E.; Fernandez-Munoz, R. (2016). A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence. Frontiers in Plant Science. 7(1732):1-16. https://doi.org/10.3389/fpls.2016.01732 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.3389/fpls.2016.01732 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 1732 es_ES
dc.relation.senia 331619 es_ES
dc.identifier.pmid 27920785 en_EN
dc.identifier.pmcid PMC5118631 en_EN
dc.contributor.funder European Commission
dc.contributor.funder European Cooperation in Science and Technology es_ES
dc.contributor.funder Netherlands Organization for Scientific Research
dc.contributor.funder Junta de Andalucía
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder European Regional Development Fund es_ES


This item appears in the following Collection(s)

Show simple item record