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Insights into the role of the berry-specific ethylene responsive factor VviERF045

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Insights into the role of the berry-specific ethylene responsive factor VviERF045

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dc.contributor.author Leida, Carmen es_ES
dc.contributor.author Dal Rì, Antonio es_ES
dc.contributor.author Dalla Costa, Lorenza es_ES
dc.contributor.author Gómez Jiménez, Maria Dolores es_ES
dc.contributor.author Pompili, Valerio es_ES
dc.contributor.author Sonego, Paolo es_ES
dc.contributor.author Engelen, Kristof es_ES
dc.contributor.author Masuero, Domenico es_ES
dc.contributor.author Rios Garcia, Gabino es_ES
dc.contributor.author Moser, Claudio es_ES
dc.date.accessioned 2017-05-15T12:38:23Z
dc.date.available 2017-05-15T12:38:23Z
dc.date.issued 2016-12
dc.identifier.issn 1664-462X
dc.identifier.uri http://hdl.handle.net/10251/81132
dc.description.abstract During grape ripening, numerous transcriptional and metabolic changes are required in order to obtain colored, sweet, and flavored berries. There is evidence that ethylene, together with other signals, plays an important role in triggering the onset of ripening. Here, we report the functional characterization of a berry-specific Ethylene Responsive Factor (ERF), VviERF045, which is induced just before véraison and peaks at ripening. Phylogenetic analysis revealed it is close to the SHINE clade of ERFs, factors involved in the regulation of wax biosynthesis and cuticle morphology. Transgenic grapevines lines overexpressing VviERF045 were obtained, in vitro propagated, phenotypically characterized, and analyzed for the content of specific classes of metabolites. The effect of VviERF045 was correlated with the level of transgene expression, with highexpressing lines showing stunted growth, discolored and smaller leaves, and a lower level of chlorophylls and carotenoids. One line with intermediate expression, L15, was characterized at the transcriptomic level and showed 573 differentially expressed genes compared to wild type plants. Microscopy and gene expression analyses point toward a major role of VviERF045 in epidermis patterning by acting on waxes and cuticle. They also indicate that VviERF045 affects phenolic secondary metabolism and induces a reaction resembling a plant immune response with modulation of receptor likekinases and pathogen related genes. These results suggest also a possible role of this transcription factor in berry ripening, likely related to changes in epidermis and cuticle of the berry, cell expansion, a decrease in photosynthetic capacity, and the activation of several defense related genes as well as from the phenylpropanoid metabolism. All these processes occur in the berry during ripening. es_ES
dc.description.sponsorship CL was supported by the Marie Curie FP7-PEOPLE-2011-CIG action program- [Graperipe project n. 303907]. Network activities have been supported by COST1106 action. en_EN
dc.language Inglés es_ES
dc.publisher Frontiers Media es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject ERF es_ES
dc.subject RNA-seq es_ES
dc.subject Over-expressing transgenic lines es_ES
dc.subject VOCs es_ES
dc.subject Wax es_ES
dc.subject Vitis vinifera es_ES
dc.title Insights into the role of the berry-specific ethylene responsive factor VviERF045 es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2016.01793
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/303907/EU en_EN
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Leida, C.; Dal Rì, A.; Dalla Costa, L.; Gómez Jiménez, MD.; Pompili, V.; Sonego, P.; Engelen, K.... (2016). Insights into the role of the berry-specific ethylene responsive factor VviERF045. Frontiers in Plant Science. 7(1793):1-17. doi:10.3389/fpls.2016.01793 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.3389/fpls.2016.01793 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 1793 es_ES
dc.relation.senia 332332 es_ES
dc.identifier.pmid 28018369 en_EN
dc.identifier.pmcid PMC5146979 en_EN


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