Guillen, I.; Gabaldón, JA.; Núnez-Delicado, E.; Puchades, R.; Maquieira Catala, Á.; Morais, S. (2011). Detection of sulphathiazole in honey samples using a lateral flow immunoassay. Food Chemistry. 129(2):624-629. https://doi.org/10.1016/j.foodchem.2011.04.080
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/81262
Title:
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Detection of sulphathiazole in honey samples using a lateral flow immunoassay
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Author:
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Guillen, Isabel
Gabaldón, José Antonio
Núnez-Delicado, Estrella
Puchades, Rosa
Maquieira Catala, Ángel
Morais, Sergi
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
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Issued date:
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Abstract:
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[EN] A lateral flow immunoassay (LFIA) was developed in the competitive reaction format and applied to test sulphathiazole (STZ) residues in honey samples. To prepare the assay test, a hapten conjugate and goat antirabbit ...[+]
[EN] A lateral flow immunoassay (LFIA) was developed in the competitive reaction format and applied to test sulphathiazole (STZ) residues in honey samples. To prepare the assay test, a hapten conjugate and goat antirabbit antiserum as capture and control reagent, respectively, were dispensed on nitrocellulose membrane. Polyclonal antiserum against sulphathiazole was conjugated to colloidal gold nanoparticles and used as the detection reagent. The visual limit of detection (cut-off value) of the sulphathiazole LFIA was 15 ng/g, reaching qualitative results within 10 min. The assay was evaluated with STZ spiked honey samples from different geographical origins (n = 25). The results were in good agreement with those obtained from liquid chromatography separation and mass spectroscopy detection (LC-MS), indicating that the LFIA test might be used as a qualitative method for the determination of sulphathiazole residues without expensive equipment. The test was also highly specific, showing no cross-reactivity to other chemically similar antibiotics. To our knowledge, this is the only work where a development of LFIA tests for the detection of sulphathiazole residues is performed. © 2011 Elsevier Ltd. All rights reserved.
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Subjects:
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Honey
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Immunoassay
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LFIA
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Sulphathiazole
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Colloidal gold nanoparticles
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Cross-reactivity
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Cut-off value
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Detection reagents
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Geographical origins
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Lateral flow immunoassay
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Mass spectroscopy
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Nitrocellulose membranes
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Polyclonal antiserum
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Qualitative method
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Visual limit
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Immunology
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Liquid chromatography
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Mass spectrometry
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Nitrocellulose
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Testing
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Food products
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Gold nanoparticle
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Sulfathiazole
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Article
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Cross reaction
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Limit of detection
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Capra hircus
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Copyrigths:
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Cerrado |
Source:
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Food Chemistry. (issn:
0308-8146
)
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DOI:
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10.1016/j.foodchem.2011.04.080
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Publisher:
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Elsevier
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Publisher version:
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http://doi.org/ 10.1016/j.jenvman.2011.04.01510.1016/j.foodchem.2011.04.080
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Project ID:
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info:eu-repo/grantAgreement/MEC//CIT-060000-2007-0057/ES/Incidencia de antibióticos en miel II/
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Thanks:
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This work was supported by a grant from Ministry of Education and Science of Spain, CIT-060000-2007-57.
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Type:
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Artículo
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