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Detection of glycosylated proteins in rabbit oviductal isthmus and uterine endometrium during early embryo development

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Detection of glycosylated proteins in rabbit oviductal isthmus and uterine endometrium during early embryo development

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dc.contributor.author Marco Jiménez, Francisco es_ES
dc.contributor.author López-Béjar, M. es_ES
dc.date.accessioned 2017-05-17T10:00:13Z
dc.date.available 2017-05-17T10:00:13Z
dc.date.issued 2013-12
dc.identifier.issn 0936-6768
dc.identifier.uri http://hdl.handle.net/10251/81271
dc.description.abstract [EN] Contents In this study, lectin histochemistry was performed on paraffin sections to compare carbohydrate expression of oviductal isthmus and uterine endometrium in rabbits during early embryo development. Rabbit embryos are surrounded not only by the zona pellucida but also by tubal secretion-derived mucinous coat material, the mucin coat. Twenty sexually mature females were euthanized at 0 (pre-ovulatory group) and 24, 72 and 96h after insemination (pseudopregnancy group). The following lectin-binding agents were used: Arachis hypogaea, Peanut (PNA) to label galactosyl (-1,3)N- acetyl-galactosamine, Dolichos biflorus Agglutinin (DBA) to label galactosyl (-1,4)N- acetyl-galactosamine, Lens curinaris (LCA) to label --mannose, -d-glucose and Pisum sativum agglutinin (PSA) to label -d-mannose, -d-glucose. Blood was collected by cardiac puncture, and direct enzyme immunoassay technique was used to measure progesterone concentration. A significant increase in total plasma progesterone concentrations was detected at 96h post-ovulation when compared with 0, 24 and 72h post-ovulation (2.9 +/- 0.5 vs 0.5 +/- 0.15, 1.6 +/- 0.5 and 1.5 +/- 0.4ng/ml, at 96h vs 0, 24 and 72h post-ovulation, respectively). No differences between pre-ovulatory and pseudopregnant females were observed for glycoprotein localization in isthmus. In contrast, in the endometrium, differences in the glycoprotein detection between pre-ovulatory and pseudopregnant stages were detected. PNA to label galactosyl (-1,3)N- acetyl-galactosamine was not detected at the pre-ovulatory stage, but its presence was detected at 24h after ovulation. Both PSA and LCA to label -d-mannose, -d-glucose were only detected at 72h after ovulation. DBA detection was similar for all stages of the reproductive cycle. Therefore, N-acetyl-galactosamine secreted from isthmus could be involved in the formation of the embryonic mucin coat. d-galactose (PNA), d-glucose and d-mannose (PSA and LCA) might be crucial for the implantation period. es_ES
dc.description.sponsorship Francisco Marco Jimernez was supported by Polytechnic University of Valencia research and development programme (3141/2008). English text version was revised by N. Macowan English Language Service. en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Reproduction in Domestic Animals es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Blastocyst coverings es_ES
dc.subject Dynamic structure es_ES
dc.subject Binding-sites es_ES
dc.subject In-vitro es_ES
dc.subject Pseudopregnant rabbits es_ES
dc.subject Delayed implantation es_ES
dc.subject Mouse blastocyst es_ES
dc.subject Cell recognition es_ES
dc.subject Heparan-sulfate es_ES
dc.subject Epithelium es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Detection of glycosylated proteins in rabbit oviductal isthmus and uterine endometrium during early embryo development es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/rda.12195
dc.relation.projectID info:eu-repo/grantAgreement/UPV//3141-2008/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Marco Jiménez, F.; López-Béjar, M. (2013). Detection of glycosylated proteins in rabbit oviductal isthmus and uterine endometrium during early embryo development. Reproduction in Domestic Animals. 48(6):967-973. doi:10.1111/rda.12195 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1111/rda.12195 es_ES
dc.description.upvformatpinicio 967 es_ES
dc.description.upvformatpfin 973 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 48 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 245527 es_ES
dc.identifier.eissn 1439-0531
dc.identifier.pmid 23758545
dc.contributor.funder Universitat Politècnica de València


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