Resumen:
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[EN] Contents The aim of this study was to investigate the effect of Taxol and Cytochalasin B on the spindle, chromosome configuration and development to blastocyst stage after parthenogenesis activation of in vivo matured ...[+]
[EN] Contents The aim of this study was to investigate the effect of Taxol and Cytochalasin B on the spindle, chromosome configuration and development to blastocyst stage after parthenogenesis activation of in vivo matured rabbit oocytes after vitrification. Oocytes were randomized into four groups: oocytes treated with Cytochalasin B or Taxol before vitrification, oocytes without treatment before vitrification and fresh oocytes. Oocytes were vitrified using Cryotop method, and meiotic spindle and chromosomal distribution were assessed with a confocal laser scanning microscopy. To determine oocyte competence, in vitro development of oocytes was assessed with parthenogenesis activation. There were no significant differences in the frequencies of normal spindle (33.0%, 31.0% and 32.6%, for non-treated, Taxol-treated and Cytochalasin B-treated oocytes, respectively) and chromosome (48.3%, 46.6% and 34.8%, for non-treated, Taxol-treated oocytes and Cytochalasin B-treated oocytes respectively) in vitrified groups, but significantly lower than those of fresh group (89.7% and 90.2%, for normal spindle and chromosome organization, respectively). No statistical differences were found in the cleavage and blastocyst development rates between non-treated and Taxol-treated oocytes (7.7% and 1.5% and 13.7% and 4.6%, for non-treated and Taxol-treated oocytes, respectively), although they were significantly lower than in the fresh group (42.3% and 32.1%, for cleavage and blastocyst development, respectively). Oocytes treated with Cytochalasin B failed to reach blastocyst stage. Normal spindle, chromosome configuration and blastocyst development of in vivo matured rabbit oocytes were damaged in vitrification, which was not improved by Taxol and Cytochalasin B pre-treatment before vitrification. Moreover, a detrimental effect on blastocyst development of Cytochalasin B pre-treatment before vitrification was observed.
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Agradecimientos:
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This work was supported by the Spanish Research Project AGL2011-30170-C02-01 Comision Interministerial de Ciencia y Tecnologia and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella ...[+]
This work was supported by the Spanish Research Project AGL2011-30170-C02-01 Comision Interministerial de Ciencia y Tecnologia and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella Jimenez was supported by a research grant from the Education Ministry of the Valencian Regional Government (programme VALi+d. ACIF/2010/262). We acknowledge the helpful technical support of Marisol Gascon Irun with confocal microscopy (Servicio de Microscopia del IBMCP, Universidad Politecnica de Valencia). English text version was revised by N. Macowan English Language Service.
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