Saenz De Juano Ribes, MDLD.; Naturil Alfonso, C.; Vicente Antón, JS.; Marco Jiménez, F. (2013). Effect of different culture systems on mRNA expression in developing rabbit embryos. Zygote. 21(1):103-109. https://doi.org/10.1017/S0967199411000414
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/81289
Title:
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Effect of different culture systems on mRNA expression in developing rabbit embryos
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Author:
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Saenz de Juano Ribes, María de los Desamparados
Naturil Alfonso, Carmen
Vicente Antón, José Salvador
Marco Jiménez, Francisco
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
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Issued date:
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Abstract:
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[EN] The rate of zygotes in vitro developed to hatched blastocyst stage was evaluated between two different commercial media (TCM-199 and Ham's F10) and two different culture systems (renewal and non-renewal single medium) ...[+]
[EN] The rate of zygotes in vitro developed to hatched blastocyst stage was evaluated between two different commercial media (TCM-199 and Ham's F10) and two different culture systems (renewal and non-renewal single medium) to determine the effects of culture conditions on rabbit embryo preimplantation development. The relative transcript abundances of OCT4, vascular endothelial growth factor (VEGF) and epidermal growth factor receptor 3 (erbB3) of resultant blastocysts were also analysed and compared with in vivo developed blastocysts. Results showed an important divergence in mRNA expression between embryos developed under in vivo and in vitro conditions despite there being no significant difference in hatching blastocyst rates between different culture systems and different media. For OCT4, transcript abundance of in vitro culture embryos differs from their in vivo chronological counterparts, but, when the medium is renewed, mRNA expression seemed similar to in vivo developed 4-day-old embryos. In addition, VEGF and erbB3 expression showed marked variation between different in vitro conditions. Therefore, the study of specific transcript abundance in rabbit blastocyst provides a more detailed description of which alterations in gene expression occur due in vitro conditions, and further studies should be carried out to reduce current limitations of long-term culture of rabbit preimplantation embryos.
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Subjects:
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Embryonic development
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Gene expression
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Ham’s F10
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Rabbit
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TCM-199
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Copyrigths:
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Cerrado |
Source:
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Zygote. (issn:
0967-1994
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DOI:
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10.1017/S0967199411000414
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Publisher:
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Cambridge University Press (CUP)
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Publisher version:
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http://doi.org/10.1017/S0967199411000414
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Project ID:
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info:eu-repo/grantAgreement/GVA//PROMETEO%2F2009%2F125/ES/Efecto de la crioconservación de embriones sobre el desarrollo y el re-establecimiento de poblaciones/
info:eu-repo/grantAgreement/MICINN//AGL2008-03274/ES/MEJORA GENETICA DEL CONEJO DE CARNE: REPRODUCCION, ESTRES Y LONGEVIDAD/
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Thanks:
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This work was supported by the Spanish Research Projects (CICYT AGL2008-03274) and the Generalitat Valenciana research program (Prometeo 2009/125). The authors thank Neil Macowan Language Services for revising the English ...[+]
This work was supported by the Spanish Research Projects (CICYT AGL2008-03274) and the Generalitat Valenciana research program (Prometeo 2009/125). The authors thank Neil Macowan Language Services for revising the English version of the manuscript.
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Type:
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Artículo
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