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Calcium concentration in vitrification medium affects the developmental competence of in vitro matured ovine oocytes

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Calcium concentration in vitrification medium affects the developmental competence of in vitro matured ovine oocytes

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dc.contributor.author Succu, Sara es_ES
dc.contributor.author Berlinguer, Fiammetta es_ES
dc.contributor.author Leoni, Giovanni G. es_ES
dc.contributor.author Bebbere, Daniela es_ES
dc.contributor.author Satta, Valentina es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.contributor.author Pasciu, Valeria es_ES
dc.contributor.author Naitana, Salvatore es_ES
dc.date.accessioned 2017-05-18T05:59:47Z
dc.date.available 2017-05-18T05:59:47Z
dc.date.issued 2011-03-01
dc.identifier.issn 0093-691X
dc.identifier.uri http://hdl.handle.net/10251/81317
dc.description.abstract [EN] The present study was designed to determine whether different calcium concentrations in the vitrification solutions could improve the developmental competence of in vitro matured ovine oocytes after cryopreservation. In vitro matured oocytes were vitrified with 16.5% ethylene glycol (EG) + 16.5% dimethylsulfoxide (DMSO) vitrification media. The base media contain different calcium concentrations, so that five experimental groups were obtained: TCM/FCS (TCM 199 + 20% fetal calf serum (FCS), [Ca2+] 9.9 mg/dl); PBS/FCS (Dulbecco Phosphate Buffered Saline (PBS) + 20% FCS, [Ca2+] 4.4 mg/dl); PBSCaMg (free)/FCS (PBS without Ca2+ and Mg2+ + 20% FCS [Ca2+] 2.2 mg/dl); PBS/BSA (PBS + 0.4% bovine serum albumin (BSA), [Ca2+] 3.2 mg/dl) and PBSCaMg (free)/BSA (PBS without Ca2+ and Mg2+ +0.4% BSA, [Ca2+] 0.4 mg/dl). After warming, the oocytes from the five experimental groups were assessed for survival, spontaneous parthenogenetic activation and developmental capacity via in vitro fertilization. Oocyte survival after vitrification procedures was better preserved in group PBSCaMg (free)/FCS compared to the others (P < 0.05). In addition, a positive correlation was found between calcium concentration in vitrification solutions and spontaneous parthenogenetic activation (correlation index 0,82; P < 0.001). Development of vitrified oocytes was significantly affected by vitrification media composition (P < 0.01). In particular, oocytes from group PBSCaMg free/FCS led to higher cleavage rates and blastocyst rate compared to the others. Our data showed that lowering calcium concentration in the vitrification medium improves the blastocyst rate of vitrified ovine oocytes, probably reducing the effect of EG + DMSO during vitrification. On the contrary, the replacement of FCS with BSA dramatically reduces the developmental potential of these oocytes. (C) 2011 Elsevier Inc. All rights reserved. es_ES
dc.description.sponsorship Dr. Sara Succu was supported by POR Fondo Sociale Europeo (FSE) 2007/2013. The authors would like to thank Regione Autonoma della Sardegna (Special Project Biodiversity) for the financial support provided and Mr. Sean Cook for the English revision of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Theriogenology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Calcium concentration es_ES
dc.subject Oocytes es_ES
dc.subject Parthenogenetic activation es_ES
dc.subject Vitrification es_ES
dc.subject Calcium es_ES
dc.subject Animal es_ES
dc.subject Animal disease es_ES
dc.subject Article es_ES
dc.subject Blastocyst es_ES
dc.subject Cryopreservation es_ES
dc.subject Culture medium es_ES
dc.subject Drug effect es_ES
dc.subject Embryo development es_ES
dc.subject Fertilization in vitro es_ES
dc.subject Growth, development and aging es_ES
dc.subject Oocyte es_ES
dc.subject Parthenogenesis es_ES
dc.subject Sheep es_ES
dc.subject Animals es_ES
dc.subject Culture Media es_ES
dc.subject Embryonic Development es_ES
dc.subject Bovinae es_ES
dc.subject Ovis es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Calcium concentration in vitrification medium affects the developmental competence of in vitro matured ovine oocytes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.theriogenology.2010.10.012
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Succu, S.; Berlinguer, F.; Leoni, GG.; Bebbere, D.; Satta, V.; Marco Jiménez, F.; Pasciu, V.... (2011). Calcium concentration in vitrification medium affects the developmental competence of in vitro matured ovine oocytes. Theriogenology. 75(4):715-721. doi:10.1016/j.theriogenology.2010.10.012 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1016/j.theriogenology.2010.10.012 es_ES
dc.description.upvformatpinicio 715 es_ES
dc.description.upvformatpfin 721 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 75 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 41227 es_ES
dc.identifier.eissn 1879-3231
dc.identifier.pmid 21144566
dc.contributor.funder European Social Fund
dc.contributor.funder Regione Autonoma della Sardegna


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