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Porcine oocyte vitrification in optimized low toxicity solution with open pulled straws

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Porcine oocyte vitrification in optimized low toxicity solution with open pulled straws

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dc.contributor.author Marco Jiménez, Francisco es_ES
dc.contributor.author Casares-Crespo, Lucía es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2017-05-18T06:27:33Z
dc.date.available 2017-05-18T06:27:33Z
dc.date.issued 2014-05
dc.identifier.issn 0967-1994
dc.identifier.uri http://hdl.handle.net/10251/81322
dc.description.abstract [EN] One of the greatest challenges for reproductive cryobiologists today is to develop an efficient cryopreservation method for human and domestic animal oocytes. The objective of the present study was to optimize a low toxicity solution called VM3 to vitrify porcine oocytes using an open pulled straw (OPS) device and to evaluate the effects on viability, chromosomal organization and cortical granules distribution. Two experiments were conducted in this study. Firstly, we determined the minimum concentration of cryoprotectant present in the VM3 solution required (7.6 M) for vitrification using an OPS device. The appearance of opacity was observed when using a cooling solution at -196 degrees C; no observable opacity was noted as vitrification. In addition, the ultrastructure of oocytes in VM3 or VM3 optimized solution was examined using cryo-scanning electron microscopy. The minimum total cryoprotectant concentration present in VM3 solution necessary for apparent vitrification was 5.6 M when combined with use of an OPS device. Use of both vitrification solutions showed a characteristic plasticized surface. In the second experiment, the relative cytotoxicity of vitrification solutions (VM3 and VM3 optimized) was studied. Oocyte viability, chromosomal organization and the cortical granules distribution were assessed by fluorescent stain. After warming, oocyte survival rate was similar to that of fresh oocytes. The vitrification process significantly reduced correct chromosomal organization and cortical granules distribution rates compared with the fresh oocytes group. However, correct chromosomal organization and cortical granules distribution rates did not differ among oocytes placed in different vitrification solutions. In conclusion, our data demonstrated that the VM3 solution can be optimized and that reduction in concentration to 5.6 M enabled vitrification of oocytes with an OPS device, however use of the VM3 optimised solution had no beneficial effect on vitrification of porcine oocytes. es_ES
dc.description.sponsorship This work was supported by funds from the Generalitat Valenciana research programme (Prometeo 2009/125) and Ajudes per a la realitzacio de projectes precompetitius d'I+D per a equips [Grants for pre-competitive R&D projects for research teams] (GVPRE/2008/206). English text version was revised by the N. Macowan English language service. en_EN
dc.language Inglés es_ES
dc.publisher Cambridge University Press (CUP) es_ES
dc.relation Generalitat Valenciana [PROMETEO 2009/125] es_ES
dc.relation Ajudes per a la realitzacio de projectes precompetitius d'I+D per a equips [GVPRE/2008/206] es_ES
dc.relation.ispartof Zygote es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject VM3 es_ES
dc.subject Ice blockers es_ES
dc.subject OPS es_ES
dc.subject Toxicity es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Porcine oocyte vitrification in optimized low toxicity solution with open pulled straws es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1017/S0967199412000524
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Marco Jiménez, F.; Casares-Crespo, L.; Vicente Antón, JS. (2014). Porcine oocyte vitrification in optimized low toxicity solution with open pulled straws. Zygote. 22(2):204-212. doi:10.1017/S0967199412000524 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1017/S0967199412000524 es_ES
dc.description.upvformatpinicio 204 es_ES
dc.description.upvformatpfin 212 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 22 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 229199 es_ES
dc.identifier.eissn 1469-8730
dc.identifier.pmid 23102007


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