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Retrozymes are a unique family of non-autonomous retrotransposons with hammerhead ribozymes that propagate in plants through circular RNAs

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Retrozymes are a unique family of non-autonomous retrotransposons with hammerhead ribozymes that propagate in plants through circular RNAs

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dc.contributor.author Cervera, Amelia es_ES
dc.contributor.author Urbina, Denisse es_ES
dc.contributor.author La Peña del Rivero, Marcos de es_ES
dc.date.accessioned 2017-05-18T08:05:45Z
dc.date.available 2017-05-18T08:05:45Z
dc.date.issued 2016-06-23
dc.identifier.issn 1474-760X
dc.identifier.uri http://hdl.handle.net/10251/81356
dc.description.abstract [EN] Background: Catalytic RNAs, or ribozymes, are regarded as fossils of a prebiotic RNA world that have remained in the genomes of modern organisms. The simplest ribozymes are the small self-cleaving RNAs, like the hammerhead ribozyme, which have been historically considered biological oddities restricted to some RNA pathogens. Recent data, however, indicate that small self-cleaving ribozymes are widespread in genomes, although their functions are still unknown. Results: We reveal that hammerhead ribozyme sequences in plant genomes form part of a new family of small non-autonomous retrotransposons with hammerhead ribozymes, referred to as retrozymes. These elements contain two long terminal repeats of approximately 350 bp, each harbouring a hammerhead ribozyme that delimitates a variable region of 600–1000 bp with no coding capacity. Retrozymes are actively transcribed, which gives rise to heterogeneous linear and circular RNAs that accumulate differentially depending on the tissue or developmental stage of the plant. Genomic and transcriptomic retrozyme sequences are highly heterogeneous and share almost no sequence homology among species except the hammerhead ribozyme motif and two small conserved domains typical of Ty3-gypsy long terminal repeat retrotransposons. Moreover, we detected the presence of RNAs of both retrozyme polarities, which suggests events of independent RNA-RNA rolling-circle replication and evolution, similarly to that of infectious circular RNAs like viroids and viral satellite RNAs. Conclusions: Our work reveals that circular RNAs with hammerhead ribozymes are frequently occurring molecules in plant and, most likely, metazoan transcriptomes, which explains the ubiquity of these genomic ribozymes and suggests a feasible source for the emergence of circular RNA plant pathogens es_ES
dc.description.sponsorship Funding for this work was provided by the Ministerio de Economia y Competitividad of Spain (grants BFU2011-23398 and BFU2014-56094-P). Support of the publication fee was provided by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation MINECO/BFU2011-23398 es_ES
dc.relation MINECO/BFU2014-56094-P es_ES
dc.relation.ispartof Genome Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Circular RNA es_ES
dc.subject LTR retrotransposons es_ES
dc.subject Viroid es_ES
dc.subject Satellite RNA es_ES
dc.title Retrozymes are a unique family of non-autonomous retrotransposons with hammerhead ribozymes that propagate in plants through circular RNAs es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s13059-016-1002-4
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Cervera, A.; Urbina, D.; La Peña Del Rivero, MD. (2016). Retrozymes are a unique family of non-autonomous retrotransposons with hammerhead ribozymes that propagate in plants through circular RNAs. Genome Biology. 17(135):1-16. doi:10.1186/s13059-016-1002-4 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1186/s13059-016-1002-4 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 17 es_ES
dc.description.issue 135 es_ES
dc.relation.senia 332030 es_ES
dc.identifier.pmid 27339130
dc.identifier.pmcid PMC4918200 en_EN
dc.contributor.funder Ministerio de Economía y Competitividad (MINECO)


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