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The Molecular Chaperone DnaK Is a Source of Mutational Robustness

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The Molecular Chaperone DnaK Is a Source of Mutational Robustness

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dc.contributor.author Aguilar-Rodríguez, Jose es_ES
dc.contributor.author Sabater-Munoz, Beatriz es_ES
dc.contributor.author Montagud-Martinez, Roser es_ES
dc.contributor.author Berlanga, Victor es_ES
dc.contributor.author Alvarez-Ponce, David es_ES
dc.contributor.author Wagner, Andreas es_ES
dc.contributor.author Fares Riaño, Mario Ali es_ES
dc.date.accessioned 2017-05-18T09:44:47Z
dc.date.available 2017-05-18T09:44:47Z
dc.date.issued 2016-09
dc.identifier.issn 1759-6653
dc.identifier.uri http://hdl.handle.net/10251/81368
dc.description.abstract [EN] Molecular chaperones, also known as heat-shock proteins, refold misfolded proteins and help other proteins reach their native conformation. Thanks to these abilities, some chaperones, such as the Hsp90 protein or the chaperonin GroEL, can buffer the deleterious phenotypic effects of mutations that alter protein structure and function. Hsp70 chaperones use a chaperoning mechanism different from that of Hsp90 and GroEL, and it is not known whether they can also buffer mutations. Here, we show that they can. To this end, we performed a mutation accumulation experiment in Escherichia coli, followed by whole-genome resequencing. Overexpression of the Hsp70 chaperone DnaK helps cells cope with mutational load and completely avoid the extinctions we observe in lineages evolving without chaperone overproduction. Additionally, our sequence data show that DnaK overexpression increases mutational robustness, the tolerance of its clients to nonsynonymous nucleotide substitutions. We also show that this elevated mutational buffering translates into differences in evolutionary rates on intermediate and long evolutionary time scales. Specifically, we studied the evolutionary rates of DnaK clients using the genomes of E. coli, Salmonella enterica, and 83 other gamma-proteobacteria. We find that clients that interact strongly with DnaK evolve faster than weakly interacting clients. Our results imply that all three major chaperone classes can buffer mutations and affect protein evolution. They illustrate how an individual protein like a chaperone can have a disproportionate effect on the evolution of a proteome. es_ES
dc.description.sponsorship The authors thank Xiaoshu Chen and Jianzhi Zhang for kindly providing us with the gene expression data. This work was supported by the Forschungskredit program of the University of Zurich (grant FK-14-076 to J.A.), the Swiss National Science Foundation (grant 31003A_146137 to A.W.), the University Priority Research Program in Evolutionary Biology at the University of Zurich (to A.W.), the Science Foundation Ireland (grant 12/IP/1673 to M.A.F.), and the Spanish Ministerio de Economia y Competitividad (grant BFU2012-36346 to M.A.F.). We posted an earlier version of this paper in bioRxiv (doi: http://dx.doi.org/10.1101/040600) on 22 February 2016.
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP) es_ES
dc.relation UZH/FK-14-076 es_ES
dc.relation SNSF/31003A_146137 es_ES
dc.relation SFI/2/IP/1673 es_ES
dc.relation MINECO/BFU2012-36346 es_ES
dc.relation.ispartof Genome Biology and Evolution es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject Molecular chaperones es_ES
dc.subject Mutational robustness es_ES
dc.subject Experimental evolution es_ES
dc.subject Protein evolution es_ES
dc.subject Escherichia coli es_ES
dc.subject DnaK es_ES
dc.title The Molecular Chaperone DnaK Is a Source of Mutational Robustness es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/gbe/evw176
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Aguilar-Rodríguez, J.; Sabater-Munoz, B.; Montagud-Martinez, R.; Berlanga, V.; Alvarez-Ponce, D.; Wagner, A.; Fares Riaño, MA. (2016). The Molecular Chaperone DnaK Is a Source of Mutational Robustness. Genome Biology and Evolution. 8(9):2979-2991. doi:10.1093/gbe/evw176 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1093/gbe/evw176 es_ES
dc.description.upvformatpinicio 2979 es_ES
dc.description.upvformatpfin 2991 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 332041 es_ES
dc.identifier.pmid 27497316
dc.identifier.pmcid PMC5630943
dc.contributor.funder University of Zurich
dc.contributor.funder Swiss National Science Foundation
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Ministerio de Economía y Competitividad


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