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The transcriptomics of an experimentally evolved plant-virus interaction

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The transcriptomics of an experimentally evolved plant-virus interaction

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dc.contributor.author Hillung, Julia es_ES
dc.contributor.author García-García, Francisco es_ES
dc.contributor.author DOPAZO, JOAQUÍN es_ES
dc.contributor.author Cuevas Torrijos, Jose Manuel es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2017-05-18T11:48:32Z
dc.date.available 2017-05-18T11:48:32Z
dc.date.issued 2016-04-26
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10251/81390
dc.description.abstract [EN] Models of plant-virus interaction assume that the ability of a virus to infect a host genotype depends on the matching between virulence and resistance genes. Recently, we evolved tobacco etch potyvirus (TEV) lineages on different ecotypes of Arabidopsis thaliana, and found that some ecotypes selected for specialist viruses whereas others selected for generalists. Here we sought to evaluate the transcriptomic basis of such relationships. We have characterized the transcriptomic responses of five ecotypes infected with the ancestral and evolved viruses. Genes and functional categories differentially expressed by plants infected with local TEV isolates were identified, showing heterogeneous responses among ecotypes, although significant parallelism existed among lineages evolved in the same ecotype. Although genes involved in immune responses were altered upon infection, other functional groups were also pervasively over-represented, suggesting that plant resistance genes were not the only drivers of viral adaptation. Finally, the transcriptomic consequences of infection with the generalist and specialist lineages were compared. Whilst the generalist induced very similar perturbations in the transcriptomes of the different ecotypes, the perturbations induced by the specialist were divergent. Plant defense mechanisms were activated when the infecting virus was specialist but they were down-regulated when infecting with generalist. es_ES
dc.description.sponsorship We thank Francisca de la Iglesia and Paula Agudo for excellent technical assistance and our labmates for useful discussions and suggestions. This work was supported by grants BFU2012-30805 from the Spanish Ministry of Economy and Competitiveness (MINECO), PROMETEOII/2014/021 from Generalitat Valenciana and EvoEvo (ICT610427) from the European Commission 7th Framework Program to SFE, and grant PROMETEOII/2014/025 to JD. JMC was supported by a JAE-doc postdoctoral contract from CSIC. JH was recipient of a predoctoral contract from MINECO. en_EN
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Tobacco-ETCH-virus es_ES
dc.subject Long-distance movement es_ES
dc.subject False discovery rate es_ES
dc.subject Plum pox virus es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject Gene expression es_ES
dc.subject Experimental evolution es_ES
dc.subject Chloroplast proteome es_ES
dc.subject ABC transporters es_ES
dc.subject Viral emergence es_ES
dc.title The transcriptomics of an experimentally evolved plant-virus interaction es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/srep24901
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/610427/EU/Evolution of Evolution/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-30805/ES/EVOLUTIONARY SYSTEMS VIROLOGY: EPISTASIS AND THE RUGGEDNESS OF ADAPTIVE LANDSCAPES, MUTATIONS IN REGULATORY SEQUENCES, AND THE HOST DETERMINANTS OF VIRAL FITNESS/
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F021/ES/Comparative systems biology of host-virus interactions/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F025/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hillung, J.; García-García, F.; Dopazo, J.; Cuevas Torrijos, JM.; Elena Fito, SF. (2016). The transcriptomics of an experimentally evolved plant-virus interaction. Scientific Reports. 6:1-19. https://doi.org/10.1038/srep24901 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1038/srep24901 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 19 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.relation.senia 323442 es_ES
dc.identifier.pmid 27113435 en_EN
dc.identifier.pmcid PMC4845063
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Generalitat Valenciana
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