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Population differentiation and selective constraints in Pelargonium line

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Population differentiation and selective constraints in Pelargonium line

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dc.contributor.author Castaño Sansano, Mª Aurora es_ES
dc.contributor.author Ruiz Garcia, Maria Leticia es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.contributor.author Hernandez Fort, Carmen es_ES
dc.date.accessioned 2017-05-18T11:52:15Z
dc.date.available 2017-05-18T11:52:15Z
dc.date.issued 2011-01
dc.identifier.issn 0168-1702
dc.identifier.uri http://hdl.handle.net/10251/81392
dc.description.abstract [EN] The genomic structure of Pelargonium line pattern virus (PLPV), a tentative member of a proposed new genus within the family Tombusviridae, has been recently determined. However, little is known about the genetic variability and population structure of this pathogen. Here, we have investigated the heterogeneity of PLPV isolates from different origins by sequence analysis of a 1817 nt fragment encompassing the movement (p7 and p9.7) and coat protein genes as well as flanking segments including the complete 3` untranslated region. We have evaluated the selective pressures operating on both viral proteins and RNA genome in order to assess the relative functional and/or structural relevance of different amino acid or nucleotide sites. The results of the study have revealed that distinct protein domains are under different selective constraints and that maintenance of certain primary and/or secondary structures in RNA regulatory sequences might be an important factor limiting viral heterogeneity. We have also performed covariation analyses to uncover potential dependencies among amino acid sites of the same protein or of different proteins. The detection of linked amino acid substitutions has permitted to draw a putative network of intra- and interprotein interactions that are likely required to accomplish the different steps of the infection cycle. Finally, we have obtained phylogenetic trees that support geographical segregation of PLPV sequences. (c) 2010 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship We are indebted to Dr. Jan van der Meij (Ball Flora Plant, Chicago) for providing PLPV isolate from USA, and to Dr. Marise Borja (Fundacion Promiva, Madrid) for the Spanish isolates and for valuable comments in the course of this work. We thank to Dr. Selma Gago (IBMCP, Valencia) for critical reading of the manuscript. We are also grateful to Dolores Arocas and Isabella Avellaneda for excellent technical assistance. This research was supported by grants BFU2006-11230 and BFU2009-11699 from Ministerio de Ciencia e Innovacion (MICINN, Spain), ACOM09/040 from Generalitat Valenciana (to C.H.), and by grant BFU2009-06993 (MICINN) (to S.F.E.). A.C. was recipient of predoctoral fellowships from the Generalitat Valenciana and from CSIC-Fundacion Bancaja and L.R. received a postdoctoral contract from the Juan de la Cierva program of MEC. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Generalitat Valenciana [ACOM09/040] es_ES
dc.relation.ispartof Virus Research es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Pelargonium line pattern virus es_ES
dc.subject Family Tombusviridae es_ES
dc.subject Genetic variability es_ES
dc.subject Selective constraints es_ES
dc.subject Covariation analysis es_ES
dc.title Population differentiation and selective constraints in Pelargonium line es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.virusres.2010.10.022
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Castaño Sansano, MA.; Ruiz Garcia, ML.; Elena Fito, SF.; Hernandez Fort, C. (2011). Population differentiation and selective constraints in Pelargonium line. Virus Research. 155(1):274-282. doi:10.1016/j.virusres.2010.10.022 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1016/j.virusres.2010.10.022 es_ES
dc.description.upvformatpinicio 274 es_ES
dc.description.upvformatpfin 282 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 155 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 211837 es_ES
dc.identifier.pmid 20979982
dc.contributor.funder Generalitat Valenciana


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