- -

Functionalization of an Antisense Small RNA

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Functionalization of an Antisense Small RNA

Mostrar el registro sencillo del ítem

Ficheros en el ítem

Thumbnail
Nombre: Rodrigo;Prakash;C ...
Tamaño: 301.1Kb
Formato: PDF
Descripción: Versión editorial
Abrir
dc.contributor.author Rodrigo Tarrega, Guillermo es_ES
dc.contributor.author Prakash, Satya es_ES
dc.contributor.author Cordero-Cucart, María Teresa es_ES
dc.contributor.author Kushwaha, Manish es_ES
dc.contributor.author Jaramillo, Alfonso es_ES
dc.date.accessioned 2017-05-18T12:45:57Z
dc.date.available 2017-05-18T12:45:57Z
dc.date.issued 2016-02-27
dc.identifier.issn 0022-2836
dc.identifier.uri http://hdl.handle.net/10251/81412
dc.description.abstract [EN] In order to explore the possibility of adding new functions to preexisting genes, we considered a framework of riboregulation. We created a new riboregulator consisting of the reverse complement of a known riboregulator. Using computational design, we engineered a cis-repressing 5' untranslated region that can be activated by this new riboregulator. As a result, both RNAs can orthogonally trans-activate translation of their cognate, independent targets. The two riboregulators can also repress each other by antisense interaction, although not symmetrically. Our work highlights that antisense small RNAs can work as regulatory agents beyond the antisense paradigm and that, hence, they could be interfaced with other circuits used in synthetic biology. (C) 2016 The Authors. Published by Elsevier Ltd. es_ES
dc.description.sponsorship We thank Senshi Shen and Mark Walsh for practical assistance in the laboratory, and William Rostain and John Duncan for critically reading the manuscript. This work was supported by the grants AGL2013-49919-EXP from Spanish Ministry of Economy and CSIC Intramural 201440I017 to G.R., and by the grants FP7-ICT-FET-610730 (EVOPROG), FP7-KBBE-613745 (PROMYS), EPSRC-BBSRC BB/M017982/1 (WISB centre) and startup allocation from the School of Life Sciences to A.J. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Molecular Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Biotechnology es_ES
dc.subject Computational design es_ES
dc.subject Information encoding es_ES
dc.subject Regulatory RNA es_ES
dc.subject Synthetic biology es_ES
dc.title Functionalization of an Antisense Small RNA es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jmb.2015.12.022
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/610730/EU/General-Purpose Programmable Evolution Machine on a Chip/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2013-49919-EXP/ES/DETECCION DE PATOGENOS Y BIOCOMPUTACION MEDIANTE CIRCUITOS REGULADORES EN PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/613745/EU/Programming synthetic networks for bio-based production of value chemicals/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//BB%2FM017982%2F1/UK/Warwick Integrative Synthetic Biology Centre/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CSIC//201440I017/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/RCUK/BBSRC/BB/M017982/1/GB/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Rodrigo Tarrega, G.; Prakash, S.; Cordero-Cucart, MT.; Kushwaha, M.; Jaramillo, A. (2016). Functionalization of an Antisense Small RNA. Journal of Molecular Biology. 428(5):889-892. https://doi.org/10.1016/j.jmb.2015.12.022 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.jmb.2015.12.022 es_ES
dc.description.upvformatpinicio 889 es_ES
dc.description.upvformatpfin 892 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 428 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 331865 es_ES
dc.identifier.pmid 26756967 en_EN
dc.identifier.pmcid PMC4819895 en_EN
dc.contributor.funder European Commission
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Consejo Superior de Investigaciones Científicas
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem