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A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection

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A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection

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dc.contributor.author Montoya Baides, Ángel es_ES
dc.contributor.author March Iborra, Mª Del Carmen es_ES
dc.contributor.author Montagut Ferizzola, Yeison Javier es_ES
dc.contributor.author Moreno Tamarit, Mª José es_ES
dc.contributor.author Manclus Ciscar, Juan José es_ES
dc.contributor.author Arnau Vives, Antonio es_ES
dc.contributor.author Jiménez Jiménez, Yolanda es_ES
dc.contributor.author Jaramillo, Marisol es_ES
dc.contributor.author Marin, Paula A. es_ES
dc.contributor.author Torres-Villa, Róbinson Alberto es_ES
dc.date.accessioned 2017-05-19T11:54:12Z
dc.date.available 2017-05-19T11:54:12Z
dc.date.issued 2017
dc.identifier.issn 1568-0266
dc.identifier.uri http://hdl.handle.net/10251/81496
dc.description.abstract Tuberculosis, one of the oldest diseases affecting human beings, is still considered as a world public health problem by the World Health Organization. Therefore, there is a need for new and more powerful analytical methods for early illness diagnosis. With this idea in mind, the development of a High Fundamental Frequency (HFF) piezoelectric immunosensor for the sensitive detection of tuberculosis was undertaken. A 38 kDa protein secreted by Mycobacterium tuberculosis was first selected as the target biomarker. Then, specific monoclonal antibodies (MAbs) were obtained. Myc-31 MAb, which showed the highest affinity to the analyte, was employed to set up a reference enzyme-linked immunosorbent assay (ELISA) with a limit of detection of 14 ng mL-1 of 38 kDa antigen. For the development of the HFF piezoelectric immunosensor, 100 MHz quartz crystals were used as transducer elements. The gold electrode surface was functionalized by covalent immobilization of the target biomarker through mixed self-assembled monolayers (mSAM) of carboxylic alkane thiols. A competitive immunoassay based on Myc-31 MAb was integrated with the transducer as sensing bio-recognition event. Reliable assay signals were obtained using low concentrations of antigen for functionalization and MAb for the competitive immunoassay. Under optimized conditions, the HFF immunosensor calibration curve for 38 kDa determination showed a limit of detection as low as 11 ng mL-1 of the biomarker. The high detectability attained by this immunosensor, in the picomolar range, makes it a promising tool for the easy, direct and sensitive detection of the tuberculosis biomarker in biological fluids such as sputum. es_ES
dc.description.sponsorship This study was supported by COLCIENCIAS (Colombia), Project no. 13335212865) and by AWSensors (Valencia, Spain). All of the authors: A. Montoya, C. March, Y.J. Montagut, M.J. Moreno, J.J. Manclus, A. Arnau, Y. Jimenez, M. Jaramillo, P. A. Marin and R.A. Torres declare that they have no conflict of interest. en_EN
dc.language Inglés es_ES
dc.publisher Bentham Science Publishers es_ES
dc.relation COLCIENCIAS (Colombia) 13335212865 es_ES
dc.relation AWSensors (Valencia, Spain) es_ES
dc.relation.ispartof Current Topics in Medicinal Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Tuberculosis es_ES
dc.subject 38 kDa Biomarker es_ES
dc.subject Monoclonal Antibody es_ES
dc.subject ELISA es_ES
dc.subject High Fundamental Frequency (HFF) es_ES
dc.subject Piezoelec- tric Immunosensor. es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.2174/1568026617666161104105210
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros de Telecomunicación - Escola Tècnica Superior d'Enginyers de Telecomunicació es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.description.bibliographicCitation Montoya Baides, Á.; March Iborra, MDC.; Montagut Ferizzola, YJ.; Moreno Tamarit, MJ.; Manclus Ciscar, JJ.; Arnau Vives, A.; Jiménez Jiménez, Y.... (2017). A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection. Current Topics in Medicinal Chemistry. 17(14):1623-1630. doi:10.2174/1568026617666161104105210 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.2174/1568026617666161104105210 es_ES
dc.description.upvformatpinicio 1623 es_ES
dc.description.upvformatpfin 1630 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 17 es_ES
dc.description.issue 14 es_ES
dc.relation.senia 332939 es_ES
dc.identifier.eissn 1873-5294


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