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Molecular mechanism for the interaction between gibberellin and brassinosteroid signaling pathways in Arabidopsis

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Molecular mechanism for the interaction between gibberellin and brassinosteroid signaling pathways in Arabidopsis

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dc.contributor.author Gallego Bartolomé, Javier es_ES
dc.contributor.author Minguet, Eugenio G. es_ES
dc.contributor.author Grau Enguix, Federico es_ES
dc.contributor.author Abbas, Mohamad es_ES
dc.contributor.author Locascio, Antonella Anna Maria es_ES
dc.contributor.author Thomas, Stephen G. es_ES
dc.contributor.author Alabadí Diego, David es_ES
dc.contributor.author Blazquez Rodriguez, Miguel Angel es_ES
dc.date.accessioned 2017-05-29T08:34:26Z
dc.date.available 2017-05-29T08:34:26Z
dc.date.issued 2012-07-14
dc.identifier.issn 0027-8424
dc.identifier.uri http://hdl.handle.net/10251/81874
dc.description.abstract [EN] Plant development is modulated by the convergence of multiple environmental and endogenous signals, and the mechanisms that allow the integration of different signaling pathways is currently being unveiled. A paradigmatic case is the concurrence of brassinosteroid (BR) and gibberellin (GA) signaling in the control of cell expansion during photomorphogenesis, which is supported by physiological observations in several plants but for which no molecular mechanism has been proposed. In this work, we show that the integration of these two signaling pathways occurs through the physical interaction between the DELLA protein GAI, which is a major negative regulator of the GA pathway, and BRASSINAZOLE RESISTANT1 (BZR1), a transcription factor that broadly regulates gene expression in response to BRs. We provide biochemical evidence, both in vitro and in vivo, indicating that GAI inactivates the transcriptional regulatory activity of BZR1 upon their interaction by inhibiting the ability of BZR1 to bind to target promoters. The physiological relevance of this interaction was confirmed by the observation that the dominant gai-1 allele interferes with BR-regulated gene expression, whereas the bzr1-1D allele displays enhanced resistance to DELLA accumulation during hypocotyl elongation. Because DELLA proteins mediate the response to multiple environmental signals, our results provide an initial molecular framework for the integration with BRs of additional pathways that control plant development. es_ES
dc.description.sponsorship We thank the Nottingham Arabidopsis Stock Centre, Tai-ping Sun, Zhi-Yong Wang, Yanhai Yin, Ana Cano-Delgado, Luis Lopez-Molina, and Francois Parcy for providing seeds or reagents; Laura Garcia-Carcel and Gaston Pizzio for help in the early stages of this work; and Salome Prat for fruitful discussions, sharing unpublished results, and careful reading of the manuscript. J.G.-B. holds a Consejo Superior de Investigaciones Cientificas Fellowship of the Joint Admissions Exercise Predoctoral Program. E. G. M. is recipient of a postdoctoral "Juan de la Cierva" contract from the Spanish Ministry of Science and Innovation. A. L. was supported in part by a fellowship of the Fondo per gli Investimenti della Ricerca di Base Progetto Giovani of the Italian Ministry of Education, University, and Research. Work in the authors' laboratory was funded by Spanish Ministry of Science and Innovation Grants BIO2007-60923, BIO2010-15071, and CSD2007-00057 and by Generalitat Valenciana Grants ACOMP/2010/190 and PROMETEO/2010/020. Rothamsted Research is funded by the Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom. en_EN
dc.language Inglés es_ES
dc.publisher National Academy of Sciences es_ES
dc.relation Ministerio de Ciencia e Innovación, Spain [BIO2007-60923] [BIO2010-15071] [CSD2007-00057] es_ES
dc.relation Generalitat Valenciana [ACOMP/2010/190] [PROMETEO 2010/020] es_ES
dc.relation Biotechnology and Biological Sciences Research Council (BBSRC), United Kingdom es_ES
dc.relation.ispartof Proceedings of the National Academy of Sciences es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cross-regulation es_ES
dc.subject Growth es_ES
dc.title Molecular mechanism for the interaction between gibberellin and brassinosteroid signaling pathways in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1073/pnas.1119992109
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gallego Bartolomé, J.; Minguet, EG.; Grau Enguix, F.; Abbas, M.; Locascio, AAM.; Thomas, SG.; Alabadí Diego, D.... (2012). Molecular mechanism for the interaction between gibberellin and brassinosteroid signaling pathways in Arabidopsis. Proceedings of the National Academy of Sciences. 109(33):13446-13451. doi:10.1073/pnas.1119992109 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1073/pnas.1119992109 es_ES
dc.description.upvformatpinicio 13446 es_ES
dc.description.upvformatpfin 13451 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 109 es_ES
dc.description.issue 33 es_ES
dc.relation.senia 233067 es_ES
dc.identifier.pmcid PMC3421204


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