Peris Chanzá, EJ.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, Á. (2013). Photoattachment of thiolated DNA probes on SU-8 spin-coated Blu-ray disk surfaces for biosensing. Journal of Materials Chemistry B. 1(45):6245-6253. https://doi.org/10.1039/c3tb21026j
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/81921
Title:
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Photoattachment of thiolated DNA probes on SU-8 spin-coated Blu-ray disk surfaces for biosensing
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Author:
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Peris Chanzá, Ernesto José
Bañuls Polo, María-José
Puchades, Rosa
Maquieira Catala, Ángel
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic
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Issued date:
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Abstract:
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[EN] A methodology to modify Blu-ray disk (BD) surfaces by spin-coating an SU-8 epoxy photoresist is evaluated, to create a hybrid material with new functionalities. The optical performance of the new film surface disk is ...[+]
[EN] A methodology to modify Blu-ray disk (BD) surfaces by spin-coating an SU-8 epoxy photoresist is evaluated, to create a hybrid material with new functionalities. The optical performance of the new film surface disk is analyzed. The light-mediated activation of epoxy-to-thiol chemistry is applied to the site-specific and covalent binding of thiol-ended oligonucleotide probes to the SU-8 disk surface. For the optimized hybridization conditions, a limit of detection of 1 pM is reached employing enzymatic development. PCR products, from a Salmonella typhimurium serotype, are detected at an amplicon concentration of 20 pM. The reading of the assays is performed using a commercial BD-player.
[-]
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Subjects:
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Compact-disk
,
Immobilization
,
Microarrays
,
Biomolecules
,
Photoresist
,
Spectroscopy
,
Fabrication
,
Expression
,
Chemistry
,
Protein
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Journal of Materials Chemistry B. (issn:
2050-750X
) (eissn:
2050-7518
)
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DOI:
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10.1039/c3tb21026j
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Publisher:
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Royal Society of Chemistry
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Publisher version:
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http://doi.org/10.1039/c3tb21026j
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Project ID:
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info:eu-repo/grantAgreement/MICINN//CTQ2010-15943/ES/ESTUDIO DE NUEVAS VIAS DE DESARROLLO DE BIOMEMS PARA SCREENING MASIVO. DEMOSTRACION DE CONCEPTO COMO HERRAMIENTA DE ANALISIS APLICABLE EN "OMICAS"./
info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F008/
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Thanks:
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This work has been funded by the projects FEDER CTQ2010-15943 (CICYT, Spain), PROMETEO 2010/008 (Generalitat Valenciana) and an interdisciplinary UPV PAID 05 - 10 grant.
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Type:
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Artículo
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