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Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA

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Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA

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dc.contributor.author Wu, Qian es_ES
dc.contributor.author Zhang, Xu es_ES
dc.contributor.author Peirats-Llobet, Marta es_ES
dc.contributor.author Belda Palazón, Borja es_ES
dc.contributor.author Wang, Xiaofeng es_ES
dc.contributor.author Cui, Shao es_ES
dc.contributor.author Yu, Xiangchun es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.contributor.author An, Chengcai es_ES
dc.date.accessioned 2017-06-07T07:22:56Z
dc.date.available 2017-06-07T07:22:56Z
dc.date.issued 2016-09
dc.identifier.issn 1040-4651
dc.identifier.uri http://hdl.handle.net/10251/82486
dc.description.abstract [EN] Abscisic acid (ABA) is an essential hormone for plant development and stress responses. ABA signaling is suppressed by clade A PP2C phosphatases, which function as key repressors of this pathway through inhibiting ABA-activated SnRK2s (SNF1-related protein kinases). Upon ABA perception, the PYR/PYL/RCAR ABA receptors bind to PP2Cs with high affinity and biochemically inhibit their activity. While thismechanismhas been extensively studied, how PP2Cs are regulated at the protein level is only starting to be explored. Arabidopsis thaliana RING DOMAIN LIGASE5 (RGLG5) belongs to a five-member E3 ubiquitin ligase family whose target proteins remain unknown. We report that RGLG5, together with RGLG1, releases the PP2C blockade of ABA signaling by mediating PP2CA protein degradation. ABA promotes the interaction of PP2CA with both E3 ligases, which mediate ubiquitination of PP2CA and are required for ABA-dependent PP2CA turnover. Downregulation of RGLG1 and RGLG5 stabilizes endogenous PP2CA and diminishes ABA-mediated responses. Moreover, the reduced response to ABA in germination assays is suppressed in the rglg1 amiR (artificial microRNA)-rglg5 pp2ca-1 triple mutant, supporting a functional link among these loci. Overall, our data indicate that RGLG1 and RGLG5 are important modulators of ABA signaling, and they unveil amechanismfor activation of the ABA pathway by controlling PP2C half-life. es_ES
dc.description.sponsorship We thank Andreas Bachmair for the rglg1 mutant, Sean R. Cutler for the pyr1 pyl1 pyl2 pyl4 seeds, Dapeng Zhang for the transgenic material harboring ABI2, Hongwei Guo and Jianmin Zhou for the pCAMBIA1300-Nluc and pCAMBIA1300-Cluc vectors, and John Olson for assistance in English editing. Work in C.A.'s laboratory was supported by grants from the National Key Basic Science "973" Program (Grant 2012CB114006), the National Natural Science Foundation (Grants 31272023, 31170231, and 90817001) of the Chinese government, and by the State Key Laboratory of Protein and Plant Gene Research, Peking University. Work in P.L.R.'s laboratory was supported by Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (Grant BIO2014-52537-R). en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation National Key Basic Science "973" Program es_ES
dc.relation Ministerio de Ciencia e Innovacion,Spanin [BIO2014-52537R] es_ES
dc.relation.ispartof Plant Cell es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject SNRK2 protein-kinases es_ES
dc.subject Thaliana L Heynh es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject Differential expression es_ES
dc.subject ABA es_ES
dc.subject 2C es_ES
dc.subject Transduction es_ES
dc.subject Degradation es_ES
dc.subject Receptor es_ES
dc.subject Stress es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1105/tpc.16.00364
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Wu, Q.; Zhang, X.; Peirats-Llobet, M.; Belda Palazón, B.; Wang, X.; Cui, S.; Yu, X.... (2016). Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA. Plant Cell. 28(9):2178-2196. doi:10.1105/tpc.16.00364 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1105/tpc.16.00364 es_ES
dc.description.upvformatpinicio 2178 es_ES
dc.description.upvformatpfin 2196 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 28 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 332396 es_ES
dc.identifier.eissn 1532-298X


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