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Modulation of ABA signaling in vivo by an engineered receptor-insensitive PP2C allele

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Modulation of ABA signaling in vivo by an engineered receptor-insensitive PP2C allele

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dc.contributor.author Dupeux, Florine es_ES
dc.contributor.author Antoni Alandes, Regina es_ES
dc.contributor.author Betz, Katja es_ES
dc.contributor.author Santiago, Julia es_ES
dc.contributor.author González Guzmán, Miguel es_ES
dc.contributor.author Rodriguez, Lesia es_ES
dc.contributor.author Rubio, Silvia es_ES
dc.contributor.author Park, Sang-Youl es_ES
dc.contributor.author Cutler, Sean R. es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.contributor.author Marquez, Jose A. es_ES
dc.date.accessioned 2017-06-07T07:33:35Z
dc.date.available 2017-06-07T07:33:35Z
dc.date.issued 2011-05
dc.identifier.issn 0032-0889
dc.identifier.uri http://hdl.handle.net/10251/82488
dc.description.abstract [EN] The plant hormone abscisic acid (ABA) plays a crucial role in the control of the stress response and the regulation of plant growth and development. ABA binding to PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS intracellular receptors leads to inhibition of key negative regulators of ABA signaling, i.e. clade A protein phosphatases type 2C (PP2Cs) such as ABA-INSENSITIVE1 and HYPERSENSITIVE TO ABA1 (HAB1), causing the activation of the ABA signaling pathway. To gain further understanding on the mechanism of hormone perception, PP2C inhibition, and its implications for ABA signaling, we have performed a structural and functional analysis of the PYR1-ABA-HAB1 complex. Based on structural data, we generated a gain-of-function mutation in a critical residue of the phosphatase, hab1(W385A), which abolished ABA-dependent receptor-mediated PP2C inhibition without impairing basal PP2C activity. As a result, hab1(W385A) caused constitutive inactivation of the protein kinase OST1 even in the presence of ABA and PYR/PYL proteins, in contrast to the receptor-sensitive HAB1, and therefore hab1(W385A) qualifies as a hypermorphic mutation. Expression of hab1(W385A) in Arabidopsis (Arabidopsis thaliana) plants leads to a strong, dominant ABA insensitivity, which demonstrates that this conserved tryptophan residue can be targeted for the generation of dominant clade A PP2C alleles. Moreover, our data highlight the critical role of molecular interactions mediated by tryptophan-385 equivalent residues for clade A PP2C function in vivo and the mechanism of ABA perception and signaling. es_ES
dc.description.sponsorship This work was supported by Ministerio de Educacion y Ciencia, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas (fellowships to R.A., J.S., L.R., and S.R.; Juan de la Cierva contract to M.-G.G.; grant no. BIO2008-00221 to P.L.R.) and by the PHC Picasso programme of the French Ministere des Affaires Etrangeres et Europeennes (grant to J.A.M.). Access to the High Throughput Crystallization Facility of the Partnership for Structural Biology in Grenoble (https://htxlab.embl.fr) was supported by the P-CUBE project funded by the European Community's Seventh Framework Programme (FP7/2007-2013; grant no. 227764). en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation MEC/BIO2008-00221
dc.relation.ispartof Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Myeloid cells irem-1 es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject Extracellular domain es_ES
dc.subject Crystal structure es_ES
dc.subject Guard-cells es_ES
dc.subject ABA es_ES
dc.subject ABI1 es_ES
dc.subject Transduction es_ES
dc.subject Stress es_ES
dc.subject Kinases es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Modulation of ABA signaling in vivo by an engineered receptor-insensitive PP2C allele es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1104/pp.110.170894
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/227764/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Dupeux, F.; Antoni Alandes, R.; Betz, K.; Santiago, J.; Gonzalez Guzman, M.; Rodriguez, L.; Rubio, S.... (2011). Modulation of ABA signaling in vivo by an engineered receptor-insensitive PP2C allele. Plant Physiology. 156(1):106-116. doi:10.1104/pp.110.170894 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1104/pp.110.170894 es_ES
dc.description.upvformatpinicio 106 es_ES
dc.description.upvformatpfin 116 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 156 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 218874 es_ES
dc.identifier.eissn 1532-2548
dc.identifier.pmcid PMC3091035
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder Consejo Superior de Investigaciones Científicas


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