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Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene beta-cyclase (Dclcyb1) expression

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Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene beta-cyclase (Dclcyb1) expression

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dc.contributor.author Moreno, J. C. es_ES
dc.contributor.author Cerda, A. es_ES
dc.contributor.author Simpson, K. es_ES
dc.contributor.author Lopez Diaz, Isabel es_ES
dc.contributor.author Carrera Bergua, Esther es_ES
dc.contributor.author Handford, M. es_ES
dc.contributor.author Stange, Christiane es_ES
dc.date.accessioned 2017-06-07T09:23:52Z
dc.date.available 2017-06-07T09:23:52Z
dc.date.issued 2016-04
dc.identifier.issn 0022-0957
dc.identifier.uri http://hdl.handle.net/10251/82503
dc.description.abstract The expression of Dclcyb1 in tobacco induces a positive feedback on the isoprenoid, carotenoid, gibberellin and chlorophyll pathways leading to an enhancement in fitness, biomass, photosynthetic efficiency and carotenoid/chlorophyll composition.Carotenoids, chlorophylls and gibberellins are derived from the common precursor geranylgeranyl diphosphate (GGPP). One of the enzymes in carotenoid biosynthesis is lycopene beta-cyclase (LCYB) that catalyzes the conversion of lycopene into beta-carotene. In carrot, Dclcyb1 is essential for carotenoid synthesis in the whole plant. Here we show that when expressed in tobacco, increments in total carotenoids, beta-carotene and chlorophyll levels occur. Furthermore, photosynthetic efficiency is enhanced in transgenic lines. Interestingly, and contrary to previous observations where overexpression of a carotenogenic gene resulted in the inhibition of the synthesis of gibberellins, we found raised levels of active GA4 and the concommitant increases in plant height, leaf size and whole plant biomass, as well as an early flowering phenotype. Moreover, a significant increase in the expression of the key carotenogenic genes, Ntpsy1, Ntpsy2 and Ntlcyb, as well as those involved in the synthesis of chlorophyll (Ntchl), gibberellin (Ntga20ox, Ntcps and Ntks) and isoprenoid precursors (Ntdxs2 and Ntggpps) was observed. These results indicate that the expression of Dclcyb1 induces a positive feedback affecting the expression of isoprenoid gene precursors and genes involved in carotenoid, gibberellin and chlorophyll pathways leading to an enhancement in fitness measured as biomass, photosynthetic efficiency and carotenoid/chlorophyll composition. es_ES
dc.description.sponsorship This work was financed by Fondecyt 1130245 (CS), FONDEF D10I1022 (CS) and Fondecyt 1140527 (MH). JCM, AC and KS were supported by Conicyt Master fellowships 2401221, 22132070 and 22130956, respectively. All authors declare to have no conflict of interest with this work. JCM designed and performed experiments and wrote the manuscript, AC performed experiments, KS performed experiments, IL-D performed gibberellin quantification and read the manuscript, EC performed gibberellin quantification, MH wrote the manuscript, and CS designed experiments and also wrote the manuscript. We would like to thank Manuel Rodriguez-Concepcion for critical reading. en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP) es_ES
dc.relation Fondecyt 1130245 es_ES
dc.relation Fondecyt 1140527 es_ES
dc.relation FONDEF D10I1022 es_ES
dc.relation Conicyt 2401221 es_ES
dc.relation Conicyt 22132070 es_ES
dc.relation Conicyt 22130956 es_ES
dc.relation.ispartof Journal of Experimental Botany es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Biomass,carotenoids,chlorophyll,gene expression,gibberellins,lycopene beta-cyclase,tobacco es_ES
dc.subject Biomass es_ES
dc.subject Carotenoids es_ES
dc.subject Chlorophyll es_ES
dc.subject Gene expression es_ES
dc.subject Gibberellins es_ES
dc.subject Lycopene beta-cyclase es_ES
dc.subject Tobacco es_ES
dc.title Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene beta-cyclase (Dclcyb1) expression es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/jxb/erw037
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Moreno, JC.; Cerda, A.; Simpson, K.; Lopez Diaz, I.; Carrera Bergua, E.; Handford, M.; Stange, C. (2016). Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene beta-cyclase (Dclcyb1) expression. Journal of Experimental Botany. 67(8):2325-2338. doi:10.1093/jxb/erw037 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1093/jxb/erw037 es_ES
dc.description.upvformatpinicio 2325 es_ES
dc.description.upvformatpfin 2338 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 67 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 331918 es_ES
dc.identifier.pmid 26893492 en_EN
dc.identifier.pmcid PMC4809289 en_EN


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