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Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection.

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Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection.

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dc.contributor.author Carbonell Olivares, Alberto es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2017-06-07T12:24:45Z
dc.date.available 2017-06-07T12:24:45Z
dc.date.issued 2016
dc.identifier.issn 1464-6722
dc.identifier.uri http://hdl.handle.net/10251/82517
dc.description.abstract [EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are two classes of artificial small RNAs (sRNAs) engineered to silence endogenous transcripts as well as viral RNAs in plants. Here, we explore the possibility of using amiRNAs and syn-tasiRNAs to specifically interfere with infections by viroids, small (250–400-nucleotide) non-coding circular RNAs with compact secondary structure infecting a wide range of plant species. The combined use of recent highthroughput methods for artificial sRNA construct generation and the Potato spindle tuber viroid (PSTVd)–Nicotiana benthamiana pathosystem allowed for the simple and time-effective screening of multiple artificial sRNAs targeting sites distributed along PSTVd RNAs of (1) or (–) polarity. The majority of amiRNAs were highly active in agroinfiltrated leaves when co-expressed with an infectious PSTVd transcript, as were syn-tasiRNAs derived from a construct including the five most effective amiRNA sequences. A comparative analysis showed that the effects of the most effective amiRNA and of the syn-tasiRNAs were similar in agroinfiltrated leaves, as well as in upper non-agroinfiltrated leaves in which PSTVd accumulation was significantly delayed. These results suggest that amiRNAs and syntasiRNAs can be used effectively to control viroid infections in plants. es_ES
dc.description.sponsorship We thank Veronica Aragones, Teresa Cordero, Arnau Puigvert and Cristina Beceiro for invaluable technical assistance. This study was supported by grants BIO2014-54269-R and AGL2013-49919-EXP from the Ministerio de Economia y Competitividad (MINECO, Spain). Alberto Carbonell was supported by an Individual Fellowship from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska Curie grant agreement No. 655841. en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Molecular Plant Pathology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Artificial microRNA es_ES
dc.subject Pathogen resistance es_ES
dc.subject PSTVd es_ES
dc.subject RNA silencing es_ES
dc.subject Small RNA es_ES
dc.subject Synthetic trans-acting siRNA es_ES
dc.subject Viroid es_ES
dc.title Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/mpp.12529
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/655841/EU en_EN
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Carbonell Olivares, A.; Daros Arnau, JA. (2016). Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection. Molecular Plant Pathology. 18(5):746-753. doi:10.1111/mpp.12529 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1111/mpp.12529 es_ES
dc.description.upvformatpinicio 746 es_ES
dc.description.upvformatpfin 753 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 331948 es_ES


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