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A nested polymerase chain reaction protocol for in planta detection of Fusicladium eriobotryae, causal agent of loquat scab

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A nested polymerase chain reaction protocol for in planta detection of Fusicladium eriobotryae, causal agent of loquat scab

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dc.contributor.author González Domínguez, Elisa es_ES
dc.contributor.author León Santana, Maela es_ES
dc.contributor.author Armengol Fortí, Josep es_ES
dc.contributor.author Berbegal Martínez, Mónica es_ES
dc.date.accessioned 2017-06-09T11:15:42Z
dc.date.available 2017-06-09T11:15:42Z
dc.date.issued 2015-05
dc.identifier.issn 0931-1785
dc.identifier.uri http://hdl.handle.net/10251/82651
dc.description.abstract [EN] Scab caused by the fungus Fusicladium eriobotryae is the most serious disease affecting loquat in Spain. Isolation of F. eriobotryae from infected tissue on culture media can be difficult due to its slow growth. A polymerase chain reaction (PCR)-based protocol was developed for F. eriobotryaespecific identification from pure culture or infected loquat tissues. The primer set was designed in the elongation factor 1-a gene (EF1-a), and speci- ficity and sensitivity for single and nested PCR were validated. The nested PCR assay resulted in 100% positive detection of F. eriobotryae in naturally and artificially infected tissues. This protocol can be useful for routine diagnosis, disease monitoring programmes and epidemiological research. es_ES
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Journal of Phytopathology es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Diagnosis es_ES
dc.subject Eriobotrya japonica es_ES
dc.subject Nested PCR es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.subject.classification ECOSISTEMAS AGROFORESTALES (UPV) es_ES
dc.title A nested polymerase chain reaction protocol for in planta detection of Fusicladium eriobotryae, causal agent of loquat scab es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/jph.12291
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.description.bibliographicCitation González Domínguez, E.; León Santana, M.; Armengol Fortí, J.; Berbegal Martinez, M. (2015). A nested polymerase chain reaction protocol for in planta detection of Fusicladium eriobotryae, causal agent of loquat scab. Journal of Phytopathology. 163(5):415-418. doi:10.1111/jph.12291 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/ 10.1111/jph.12291 es_ES
dc.description.upvformatpinicio 415 es_ES
dc.description.upvformatpfin 418 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 163 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 284972 es_ES
dc.identifier.eissn 1439-0434


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