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Detection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR

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Detection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR

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dc.contributor.author Herrera Vásquez, José Ángel es_ES
dc.contributor.author Rubio, Luis es_ES
dc.contributor.author Alfaro Fernández, Ana Olvido es_ES
dc.contributor.author Debreczeni, Diana Elvira es_ES
dc.contributor.author Font San Ambrosio, Maria Isabel es_ES
dc.contributor.author Falk, Bryce W. es_ES
dc.contributor.author Ferriol, Inmaculada es_ES
dc.date.accessioned 2017-06-14T09:27:55Z
dc.date.available 2017-06-14T09:27:55Z
dc.date.issued 2015-09-01
dc.identifier.issn 0166-0934
dc.identifier.uri http://hdl.handle.net/10251/82828
dc.description.abstract [EN] Tomato torrado virus (ToTV) causes serious damage to the tomato industry and significant economic losses. A quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) method using primers and a specific TaqMan (R) MGB probe for ToTV was developed for sensitive detection and quantitation of different ToTV isolates. A standard curve using RNA transcripts enabled absolute quantitation, with a dynamic range from 10(4) to 10(10) ToTV RNA copies/ng of total RNA. The specificity of the RT-qPCR was tested with twenty-three ToTV isolates from tomato (Solanum lycopersicum L), and black nightshade (Solanum nigrum L) collected in Spain, Australia, Hungary and France, which covered the genetic variation range of this virus. This new RT-qPCR assay enables a reproducible, sensitive and specific detection and quantitation of ToTV, which can be a valuable tool in disease management programs and epidemiological studies. (C) 2015 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship This research was partially funded by grant RTA2013-00047-C02 from the Instituto Nacional de Investigaciones Agrarias (INIA) of Spain and supported in part by grant SCB11058 from the California Department of Food and Agriculture (USA). Dr. J.A. Herrera-Vasquez was supported by the Secretaria Nacional de Ciencia, Tecnologia e Innovacion (SENACYT) of Panama through the project 31-2011-4-ITE-002, and a National Research Investigator award (SNI) from the SENACYT during a visit at the Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain. We thank Dr. B. Gabor (Csongrad Megyei MgSzH, Hungary) and Dr. A. Forray (Floraton Kft., Hungary) for providing the Hungarian isolates of ToTV, Dr. M. Turina (Istituto per la Protezione Sostenibile delle Piante, Sez. di Torino, CNR, Turin, Italy) for providing the ToANV isolate, Dr. R. Gilbertson (UC Davis, USA) for providing the ToChSV isolate, Dr. E. Verdin (INRA, France) for providing the ToTV French isolate and Dr. C. Gambley (Department of Agriculture, Fisheries and Forestry Ecosciences Precint, Australia) for providing the Australian ToTV isolates. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Virological Methods es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Torradovirus es_ES
dc.subject Secoviridae es_ES
dc.subject Detection es_ES
dc.subject Diagnosis es_ES
dc.subject Quantitative RT-PCR es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Detection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jviromet.2015.04.029
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2013-00047-C02/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CDFA//SCB11058/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/SENACYT//31-2011-4-ITE-002/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Herrera Vásquez, JÁ.; Rubio, L.; Alfaro Fernández, AO.; Debreczeni, DE.; Font San Ambrosio, MI.; Falk, BW.; Ferriol, I. (2015). Detection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR. Journal of Virological Methods. 221:90-94. https://doi.org/10.1016/j.jviromet.2015.04.029 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1016/j.jviromet.2015.04.029 es_ES
dc.description.upvformatpinicio 90 es_ES
dc.description.upvformatpfin 94 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 221 es_ES
dc.relation.senia 308427 es_ES
dc.identifier.eissn 1879-0984
dc.identifier.pmid 25956672
dc.contributor.funder California Department of Food and Agriculture es_ES
dc.contributor.funder Secretaría Nacional de Ciencia, Tecnología e Innovación, Panamá es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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