Mostrar el registro sencillo del ítem
dc.contributor.author | Alfaro Fernández, Ana Olvido | es_ES |
dc.contributor.author | Mai Abdala, Ali | es_ES |
dc.contributor.author | Abdelraheem, Fadia Mobarak | es_ES |
dc.contributor.author | Saeed, Ebrahim Abd Elhameen | es_ES |
dc.contributor.author | Font San Ambrosio, Maria Isabel | es_ES |
dc.date.accessioned | 2017-06-14T10:23:33Z | |
dc.date.available | 2017-06-14T10:23:33Z | |
dc.date.issued | 2012-08 | |
dc.identifier.issn | 0929-1873 | |
dc.identifier.uri | http://hdl.handle.net/10251/82832 | |
dc.description.abstract | [EN] In January 2011, symptomatic chickpea and faba bean plants were observed in fields located in the Gezira state (Sudan). Faba bean plants showed yellowing and stunting, whereas chickpea plants presented yellowing, reddening and little leaves. The disease etiology was investigated using nested polymerase chain reaction (PCR) with phytoplasma-specific primers which amplify a fragment of the 16S rRNA gene. Sequencing and restriction fragment length polymorphism (RFLP) analyses revealed that the tested phytoplasmas belonged to the group 16SrII. Phylogenetic analyses of the 16S rRNA gene of the obtained sequences indicated that the chickpea and faba bean phytoplasmas from Sudan were more closely related to the phytoplasmas subgroup 16SrII-D. To our knowledge, this is the first report of phytoplasmas from the group 16SrII-D infecting chickpea in Sudan, and faba bean worldwide. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Springer Verlag (Germany) | es_ES |
dc.relation.ispartof | European Journal of Plant Pathology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Cicer arietinum | es_ES |
dc.subject | Group 16SrII | es_ES |
dc.subject | PCR | es_ES |
dc.subject | RFLP. | es_ES |
dc.subject | Vicea faba | es_ES |
dc.subject.classification | PRODUCCION VEGETAL | es_ES |
dc.title | Molecular identification of 16SrII-D subgroup phytoplasmas associated with chickpea and faba bean in Sudan | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1007/s10658-012-9975-7 | |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Alfaro Fernández, AO.; Mai Abdala, A.; Abdelraheem, FM.; Saeed, EAE.; Font San Ambrosio, MI. (2012). Molecular identification of 16SrII-D subgroup phytoplasmas associated with chickpea and faba bean in Sudan. European Journal of Plant Pathology. 133(4):791-795. https://doi.org/10.1007/s10658-012-9975-7 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1007/s10658-012-9975-7 | es_ES |
dc.description.upvformatpinicio | 791 | es_ES |
dc.description.upvformatpfin | 795 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 133 | es_ES |
dc.description.issue | 4 | es_ES |
dc.relation.senia | 235546 | es_ES |
dc.description.references | Akthar, K. P., Shah, T. M., Atta, B. M., Dickinson, M., Jamil, F. F., Haq, M. A., et al. (2008). Natural occurrence of phytoplasma associated with chickpea phyllody disease in Pakistan—a new record. Plant Pathology, 57, 771. | es_ES |
dc.description.references | Bertaccini, A., & Duduk, B. (2009). Phytoplasma and phytoplasma diseases: a review of recent research. Phytopathologia Mediterranea, 48, 355–378. | es_ES |
dc.description.references | Deng, S., & Hiruki, C. (1991). Genetic relatedness between two non-culturable micoplasma-like organisms revealed by nucleic acid hybridization and polymerase chain reaction. Phytopathology, 81, 1475–1479. | es_ES |
dc.description.references | Green, M. J., & Thompson, D. A. (1999). Easy and efficient DNA extraction from woody plants for the detection of phytoplasmas by polymerase chain reaction. Plant Disease, 83, 482–485. | es_ES |
dc.description.references | Gundersen, D. E., & Lee, I. M. (1996). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea, 35, 144–151. | es_ES |
dc.description.references | IRPCM. (2004). “Candidatus Phytoplasma”, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. International Journal of Systematic and Evolutionary Microbiology, 54, 1243–1255. | es_ES |
dc.description.references | Jones, P., & Cockbain, A. J. (1984). Association of a mycoplasma-like organism with broad bean phyllody in the Sudan. Plant Pathology, 33, 599–602. | es_ES |
dc.description.references | Khan, A. J., Botti, S., Al-Subhi, A. M., Gundersen-Rindal, D. E., & Bertaccini, A. F. (2002). Molecular identification of a new phytoplasma associated with Alfalfa witches’—broom in Oman. Phytopathology, 92, 1038–1047. | es_ES |
dc.description.references | Lee, I.-M., Gundersen-Rindal, D. E., Davis, R. E., & Bartoszyk, I. M. (1998). Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein genes sequences. International Journal of Systematic Bacteriology, 48, 1153–1169. | es_ES |
dc.description.references | Lee, I. M., Davis, R. E., & Gundersen-Rindal, D. E. (2000). Phytoplasma: phytopathogenic mollicutes. Annual Review of Microbiology, 5, 221–255. | es_ES |
dc.description.references | Schneider, B., Seemüller, E., Smart, C. D., & Kirkpatrick, B. C. (1995). Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In S. Razin & J. G. Tully (Eds.), Molecular and diagnostic procedures in Micoplasmology, vol. 1 (pp. 369–380). San Diego: Academic Press. | es_ES |
dc.description.references | Schneider, B., Gibb, K. S., & Seemüller, E. (1997). Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas. Microbiology, 143, 3381–3389. | es_ES |
dc.description.references | Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739. | es_ES |
dc.description.references | White, D. T., Blackall, L. L., Scott, T., & Walsh, K. B. (1998). Phylogenetic positions of phytoplasmas associated with dieback, yellow crinkle and mosaic diseases of papaya, and their proposed inclusion in “Candidatus Phytoplasma australiense” and a new taxon “Candidatus Phytoplasma australasia”. International Journal of Systematic Bacteriology, 48, 941–951. | es_ES |