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Optimal viral strategies for bypassing RNA silencing

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Optimal viral strategies for bypassing RNA silencing

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dc.contributor.author Rodrigo Tarrega, Guillermo es_ES
dc.contributor.author Carrera, Javier es_ES
dc.contributor.author Jaramillo, Alfonso es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2017-06-19T07:06:34Z
dc.date.available 2017-06-19T07:06:34Z
dc.date.issued 2011-02-06
dc.identifier.issn 1742-5689
dc.identifier.uri http://hdl.handle.net/10251/83125
dc.description.abstract [EN] The RNA silencing pathway constitutes a defence mechanism highly conserved in eukaryotes, especially in plants, where the underlying working principle relies on the repressive action triggered by the intracellular presence of double-stranded RNAs. This immune system performs a post-transcriptional suppression of aberrant mRNAs or viral RNAs by small interfering RNAs (siRNAs) that are directed towards their target in a sequence-specific manner. However, viruses have evolved strategies to escape from silencing surveillance while promoting their own replication. Several viruses encode suppressor proteins that interact with different elements of the RNA silencing pathway and block it. The different suppressors are not phylogenetically nor structurally related and also differ in their mechanism of action. Here, we adopt a model-driven forward-engineering approach to understand the evolution of suppressor proteins and, in particular, why viral suppressors preferentially target some components of the silencing pathway. We analysed three strategies characterized by different design principles: replication in the absence of a suppressor, suppressors targeting the first protein component of the pathway and suppressors targeting the siRNAs. Our results shed light on the question of whether a virus must opt for devoting more time into transcription or into translation and on which would be the optimal step of the silencing pathway to be targeted by suppressors. In addition, we discussed the evolutionary implications of such designing principles. © 2010 The Royal Society. es_ES
dc.description.sponsorship This work was supported by the Spanish Ministerio de Ciencia e Innovacion grants BFU2009-06993 to S.F E. and TIN-2006-12860 to A.J, and by FP6-NEST-043340 (BioModularH2), FP7-ICT-043338 (Bactocom), FP7-KBBE-212894 (Tarpol), the Structural Funds of the European Regional Development Fund, the ATIGE-Genopole and the Foundation pour la Recherche Medicale grants (all to A.J.). J.C, G.R. and A.J. also acknowledge the HPC-Europa programme (RII3-CT-2003-506079). G.R. was supported by a graduate fellowship from the Generalitat Valenciana and an EMBO Short-term fellowship. S.F.E. also acknowledges support from the Santa Fe Institute. en_EN
dc.language Inglés es_ES
dc.publisher Royal Society, The es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//BFU2009-06993/ES/Biologia Evolutiva Y De Sistemas De La Emergencia De Fitovirus De Rna/ es_ES
dc.relation EU/FP6-NEST-043340 es_ES
dc.relation MICINN/TIN-2006-12860
dc.relation EU/RII3-CT-2003-506079
dc.relation.ispartof Interface es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject RNA silencing es_ES
dc.subject Silencing suppression es_ES
dc.subject Systems and synthetic biology es_ES
dc.subject Transcription-translation tradeoff es_ES
dc.subject Virus evolution es_ES
dc.subject Virus-host interaction es_ES
dc.subject Defence mechanisms es_ES
dc.subject Design Principles es_ES
dc.subject Designing principle es_ES
dc.subject Double-stranded RNA es_ES
dc.subject Immune systems es_ES
dc.subject Mechanism of action es_ES
dc.subject Model-driven es_ES
dc.subject Post-transcriptional es_ES
dc.subject Protein components es_ES
dc.subject RNA silencing pathway es_ES
dc.subject Sequence-specific manner es_ES
dc.subject Small interfering RNA es_ES
dc.subject Synthetic biology es_ES
dc.subject Viral RNA es_ES
dc.subject Working principles es_ES
dc.subject Biology es_ES
dc.subject Optimization es_ES
dc.subject Proteins es_ES
dc.subject Transcription es_ES
dc.subject Viruses es_ES
dc.subject RNA es_ES
dc.subject RNA induced silencing complex es_ES
dc.subject Article es_ES
dc.subject Gene silencing es_ES
dc.subject Mathematical model es_ES
dc.subject Nonhuman es_ES
dc.subject RNA transcription es_ES
dc.subject RNA translation es_ES
dc.subject Suppressor gene es_ES
dc.subject Virus es_ES
dc.subject Virus cell interaction es_ES
dc.subject Virus replication es_ES
dc.subject Evolution, Molecular es_ES
dc.subject Models, Genetic es_ES
dc.subject RNA Interference es_ES
dc.subject RNA Viruses es_ES
dc.subject RNA, Small Interfering es_ES
dc.subject Suppression, Genetic es_ES
dc.subject Viral Proteins es_ES
dc.subject Eukaryota es_ES
dc.title Optimal viral strategies for bypassing RNA silencing es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1098/rsif.2010.0264
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/248919/EU/Bacterial Computing with Engineered Populations/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/212894/EU/Targeting environmental pollution with engineered microbial systems á la carte/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Rodrigo Tarrega, G.; Carrera, J.; Jaramillo, A.; Elena Fito, SF. (2011). Optimal viral strategies for bypassing RNA silencing. Interface. 8(55):257-268. https://doi.org/10.1098/rsif.2010.0264 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1098/rsif.2010.0264 es_ES
dc.description.upvformatpinicio 257 es_ES
dc.description.upvformatpfin 268 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 55 es_ES
dc.relation.senia 218175 es_ES
dc.identifier.pmid 20573628 en_EN
dc.identifier.pmcid PMC3033024
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Generalitat Valenciana


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