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INDEHISCENT and SPATULA interact to specify carpel and valve margin tissue and thus promote seed dispersal in Arabidopsis

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INDEHISCENT and SPATULA interact to specify carpel and valve margin tissue and thus promote seed dispersal in Arabidopsis

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dc.contributor.author Girin, T. es_ES
dc.contributor.author Paicu, T. es_ES
dc.contributor.author Stephenson, P. es_ES
dc.contributor.author Fuentes, S. es_ES
dc.contributor.author Körner, E. es_ES
dc.contributor.author O'Brien, M. es_ES
dc.contributor.author Sorefan, K. es_ES
dc.contributor.author Wood, T. A. es_ES
dc.contributor.author Balanzá Pérez, Vicente es_ES
dc.contributor.author Ferrandiz Maestre, Cristina es_ES
dc.contributor.author Smyth, D.R. es_ES
dc.contributor.author Østergaard, L. es_ES
dc.date.accessioned 2017-06-21T11:54:08Z
dc.date.available 2017-06-21T11:54:08Z
dc.date.issued 2011-10
dc.identifier.issn 1040-4651
dc.identifier.uri http://hdl.handle.net/10251/83364
dc.description.abstract [EN] Structural organization of organs in multicellular organisms occurs through intricate patterning mechanisms that often involve complex interactions between transcription factors in regulatory networks. For example, INDEHISCENT (IND), a basic helix-loop-helix (bHLH) transcription factor, specifies formation of the narrow stripes of valve margin tissue, where Arabidopsis thaliana fruits open on maturity. Another bHLH transcription factor, SPATULA (SPT), is required for reproductive tissue development from carpel margins in the Arabidopsis gynoecium before fertilization. Previous studies have therefore assigned the function of SPT to early gynoecium stages and IND to later fruit stages of reproductive development. Here we report that these two transcription factors interact genetically and via protein-protein contact to mediate both gynoecium development and fruit opening. We show that IND directly and positively regulates the expression of SPT, and that spt mutants have partial defects in valve margin formation. Careful analysis of ind mutant gynoecia revealed slight defects in apical tissue formation, and combining mutations in IND and SPT dramatically enhanced both single-mutant phenotypes. Our data show that SPT and IND at least partially mediate their joint functions in gynoecium and fruit development by controlling auxin distribution and suggest that this occurs through cooperative binding to regulatory sequences in downstream target genes. es_ES
dc.description.sponsorship We thank Steve Penfield for spt-11 and spt-12 mutant seeds, Michael Groszmann for the IND genomic clone, 35S:SPT plants, and for useful discussions, Charlie Scutt for critical comments on the manuscript, Nicolas Arnaud for useful discussions, and Martin F. Yanofsky and Kristina Gremski for sharing unpublished observations and useful discussions. We are grateful to Andrew Davis, Sue Bunnewell, and Kim Findlay at the John Innes Centre for assistance with photography, transmission electron microscopy, and scanning electron microscopy and to Dian Guan for constructing the double G-box vector for the yeast one-hybrid experiment. This work was funded by grants from the Biotechnological and Biological Science Research Council (BB/D018005/1 to L. O.), from the Australian Research Council (A19927094 to D. R. S.), from the Spanish Government (BIO2009-09920 to C. F.), and by a Marie Curie Fellowship (MEST-CT-2005-019727 to S.F.). en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation Biotechnological and Biological Science Research Council (BB/D018005/1 to L. O.) es_ES
dc.relation Australian Research Council (A19927094 to D. R. S.) es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//BIO2009-09920/ES/Conservacion De Los Modelos Geneticos Que Explican La Morfogenesis Del Carpelo Y El Fruto En Las Eudicotiledoneas/ es_ES
dc.relation.ispartof Plant Cell es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Arabidopsis protein es_ES
dc.subject Basic helix loop helix transcription factor es_ES
dc.subject INDEHISCENT protein, Arabidopsis es_ES
dc.subject Indoleacetic acid derivative es_ES
dc.subject SPT protein, Arabidopsis es_ES
dc.subject Arabidopsis es_ES
dc.subject Article es_ES
dc.subject Cytology es_ES
dc.subject Flower es_ES
dc.subject Fruit es_ES
dc.subject Gene expression regulation es_ES
dc.subject Genetics es_ES
dc.subject Growth, development and aging es_ES
dc.subject Metabolism es_ES
dc.subject Mutation es_ES
dc.subject Phenotype es_ES
dc.subject Physiology es_ES
dc.subject Plant seed es_ES
dc.subject Protein analysis es_ES
dc.subject Regulatory sequence es_ES
dc.subject Reproduction es_ES
dc.subject Seed dispersal es_ES
dc.subject Arabidopsis Proteins es_ES
dc.subject Basic Helix-Loop-Helix Transcription Factors es_ES
dc.subject Flowers es_ES
dc.subject Gene Expression Regulation, Plant es_ES
dc.subject Indoleacetic Acids es_ES
dc.subject Protein Interaction Mapping es_ES
dc.subject Regulatory Sequences, Nucleic Acid es_ES
dc.subject Seeds es_ES
dc.title INDEHISCENT and SPATULA interact to specify carpel and valve margin tissue and thus promote seed dispersal in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1105/tpc.111.090944
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Girin, T.; Paicu, T.; Stephenson, P.; Fuentes, S.; Körner, E.; O'brien, M.; Sorefan, K.... (2011). INDEHISCENT and SPATULA interact to specify carpel and valve margin tissue and thus promote seed dispersal in Arabidopsis. Plant Cell. 23(10):3641-3653. https://doi.org/10.1105/tpc.111.090944 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1105/tpc.111.090944 es_ES
dc.description.upvformatpinicio 3641 es_ES
dc.description.upvformatpfin 3653 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 23 es_ES
dc.description.issue 10 es_ES
dc.relation.senia 211467 es_ES
dc.identifier.eissn 1532-298X
dc.identifier.pmid 21990939
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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