- -

Functional analyses of AGAMOUS family members in Nicotiana benthamiana clarify the evolution of early and late roles of C-function genes in eudicots

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Functional analyses of AGAMOUS family members in Nicotiana benthamiana clarify the evolution of early and late roles of C-function genes in eudicots

Show simple item record

Files in this item

dc.contributor.author Fourquin, Chloe es_ES
dc.contributor.author Ferrandiz Maestre, Cristina es_ES
dc.date.accessioned 2017-06-21T12:01:27Z
dc.date.available 2017-06-21T12:01:27Z
dc.date.issued 2012-09
dc.identifier.issn 0960-7412
dc.identifier.uri http://hdl.handle.net/10251/83365
dc.description.abstract [EN] The C-function, according to the ABC model of floral organ identity, is required for stamen and carpel development and to provide floral meristem determinacy. Members of the AG lineage of the large MADS box gene family specify the C-function in a broadly conserved manner in angiosperms. In core eudicots, two sub-lineages co-exist, euAG and PLE, which have been extensively characterized in Antirrhinum majus and Arabidopsis thaliana, where strong sub-functionalization has led to highly divergent contributions of the respective paralogs to the C-function. Various scenarios have been proposed to reconstruct the evolutionary history of the euAG and PLE lineages in eudicots, but detailed functional analyses of the roles of these genes in additional representative species to validate evolutionary hypotheses are scarce. Here, we report functional characterization of euAG- and PLE-like genes in Nicotiana benthamiana through expression analyses and phenotypic characterization of the defects caused by their specific down-regulation. We show that both paralogs redundantly contribute to the C-function in this species, providing insights on the likely evolution of these gene lineages following divergence of the major groups within the eudicots (rosids and asterids). Moreover, we have demonstrated a conserved role for the PLE-like genes in controlling fruit dehiscence, which strongly supports the ancestral role of PLE-like genes in late fruit development and suggests a common evolutionary origin of late developmental processes in dry (dehiscent) and fleshy (ripening) fruits. es_ES
dc.description.sponsorship We thank Rafael Martinez-Pardo and Eugenio Grau for technical support, Amy Litt (New York Botanical Gradens, Bronx, NY, USA) for providing VIGS plasmids, Eugenio Minguet for help with the LFY matrix and Morpheus tool, and Francisco Madueno, Monica Colombo and Barbara Ambrose (NYBG) for critical reading of the manuscript. The work was funded by grant BIO2009-09920 from the Spanish Ministerio de Ciencia e Innovacion to C.F. en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation Ministerio de Ciencia e Innovación, Spain [BIO2009-09920] es_ES
dc.relation.ispartof Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject C-function es_ES
dc.subject AGAMOUS es_ES
dc.subject SHATTERPROOF es_ES
dc.subject Flower development es_ES
dc.subject Dehiscence es_ES
dc.subject VIGS, es_ES
dc.subject Nicotiana benthamiana es_ES
dc.subject Fruit development es_ES
dc.title Functional analyses of AGAMOUS family members in Nicotiana benthamiana clarify the evolution of early and late roles of C-function genes in eudicots es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1365-313X.2012.05046.x
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Fourquin, C.; Ferrandiz Maestre, C. (2012). Functional analyses of AGAMOUS family members in Nicotiana benthamiana clarify the evolution of early and late roles of C-function genes in eudicots. Plant Journal. 71(6):990-1001. doi:10.1111/j.1365-313X.2012.05046.x es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1111/j.1365-313X.2012.05046.x es_ES
dc.description.upvformatpinicio 990 es_ES
dc.description.upvformatpfin 1001 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 71 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 232823 es_ES
dc.identifier.pmid 22563981


This item appears in the following Collection(s)

Show simple item record