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Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering

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Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering

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dc.contributor.author Hueso Estornell, Leandro es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2017-06-22T09:47:27Z
dc.date.available 2017-06-22T09:47:27Z
dc.date.issued 2012
dc.identifier.isbn 978-1-61779-557-2
dc.identifier.isbn 978-1-61779-558-9
dc.identifier.issn 1064-3745
dc.identifier.uri http://hdl.handle.net/10251/83432
dc.description.abstract [EN] MultiSite Gateway cloning techniques based on homologous recombination facilitate the combinatorial assembly of basic genetic pieces (i.e., promoters, CDS, and terminators) into gene expression or gene silencing cassettes. pENFRUIT is a collection of MultiSite Triple Gateway Entry vectors dedicated to genetic engineering in fruits. It comprises a number of fruit-operating promoters as well as C-terminal tags adapted to the Gateway standard. In this way, flanking regulatory/labeling sequences can be easily Gateway-assembled with a given gene of interest for its ectopic expression or silencing in fruits. The resulting gene constructs can be analyzed in stable transgenic plants or in transient expression assays, the latter allowing fast testing of the increasing number of combinations arising from MultiSite methodology. A detailed description of the use of MultiSite cloning methodology for the assembly of pENFRUIT elements is presented. es_ES
dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Education (Ramón y Cajal program, BIO2010-15384 and BIO2008-03434 projects), the European Commission (program I3P and EU-SOL project), and Fundación Genoma España (ESP-SOL project).
dc.language Inglés es_ES
dc.publisher Humana Press es_ES
dc.relation.ispartof Transgenic Plants: Methods and Protocols es_ES
dc.relation.ispartofseries Methods in Molecular Biology;847
dc.rights Reserva de todos los derechos es_ES
dc.subject Gateway cloning es_ES
dc.subject Gene expression es_ES
dc.subject Gene silencing es_ES
dc.subject HpRNAi es_ES
dc.subject Transient expression es_ES
dc.subject Agroinjection es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering es_ES
dc.type Artículo es_ES
dc.type Capítulo de libro es_ES
dc.identifier.doi 10.1007/978-1-61779-558-9_28
dc.relation.projectID info:eu-repo/grantAgreement/MEC//BIO2005-01015/ES/PRODUCCION DE PROTEINAS INMUNOTERAPEUTICAS DE APLICACION ORAL EN FRUTOS DE TOMATE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/268465/EU/Real-time experimental study of pattern selection dynamics in eutectic solidification/
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2008-03434/ES/EL FRUTO DE TOMATE COMO BIOFACTORIA DE PROTEINAS INMUNOTERAPEUTICAS ORALES: PRODUCCION DE ANTICUERPOS FRENTE A ROTAVIRUS/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hueso Estornell, L.; Granell Richart, A.; Orzáez Calatayud, DV. (2012). Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering. Transgenic Plants: Methods and Protocols. 847:351-368. https://doi.org/10.1007/978-1-61779-558-9_28 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/978-1-61779-558-9_28 es_ES
dc.description.upvformatpinicio 351 es_ES
dc.description.upvformatpfin 368 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 847 es_ES
dc.relation.senia 232822 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
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