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Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications

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Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications

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dc.contributor.author Juarez, Paloma es_ES
dc.contributor.author Virdi, Vikram es_ES
dc.contributor.author Depicker, Ann es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2017-06-22T10:08:28Z
dc.date.available 2017-06-22T10:08:28Z
dc.date.issued 2016-09
dc.identifier.issn 1467-7644
dc.identifier.uri http://hdl.handle.net/10251/83437
dc.description.abstract [EN] Although plant expression systems used for production of therapeutic proteins have the advantage of being scalable at a low price, the downstream processing necessary to obtain pure therapeutic molecules is as expensive as for the traditional Chinese hamster ovary (CHO) platforms. However, when edible plant tissues (EPTs) are used, there is no need for exhaustive purification, because they can be delivered orally as partially purified formulations that are safe for consumption. This economic benefit is especially interesting when high doses of recombinant proteins are required throughout the treatment/prophylaxis period, as is the case for antibodies used for oral passive immunization (OPI). The secretory IgA (SIgA) antibodies, which are highly abundant in the digestive tract and mucosal secretions, and thus the first choice for OPI, have only been successfully produced in plant expression systems. Here, we cover most of the up-todate examples of EPT-produced pharmaceuticals, including two examples of SIgA aimed at oral delivery. We describe the benefits and drawbacks of delivering partially purified formulations and discuss a number of practical considerations and criteria to take into account when using plant expression systems, such as subcellular targeting, protein degradation, glycosylation patterns and downstream strategies, all crucial for improved yield, high quality and low cost of the final product. es_ES
dc.description.sponsorship The authors would like to thank Annick Bleys for assistance with the manuscript preparation. P.J. would like to express gratitude towards the Spanish Ministry of Economy and Competiveness for her FPU fellowship and towards the International Society for Plant Molecular Farming for their generous bursaries for attending the PBVAB 2015. This work was supported by grants from Research Foundation Flanders (FWO project G0C9714N), from the European Commission (H2020-MSCA-IF-2014 Proposal 658701-ImmunoFarm) and from the Spanish Ministry of Economy and Competiveness (Plan Nacional I+D Grant BIO2013-42193R).
dc.language Inglés es_ES
dc.publisher Wiley Open Access es_ES
dc.relation MINECO/BIO2013-42193R es_ES
dc.relation.ispartof Plant Biotechnology Journal es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Molecular farming es_ES
dc.subject Edible es_ES
dc.subject Antibody es_ES
dc.subject Therapeutic protein es_ES
dc.subject Oral passive immunization es_ES
dc.subject Mucosae es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications es_ES
dc.type Artículo es_ES
dc.type Reseña es_ES
dc.identifier.doi 10.1111/pbi.12541
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/658701/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Juarez, P.; Virdi, V.; Depicker, A.; Orzáez Calatayud, DV. (2016). Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications. Plant Biotechnology Journal. 14(9):1791-1799. doi:10.1111/pbi.12541 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.1111/pbi.12541 es_ES
dc.description.upvformatpinicio 1791 es_ES
dc.description.upvformatpfin 1799 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 331812 es_ES
dc.identifier.eissn 1467-7652
dc.identifier.pmid 26873071 en_EN
dc.identifier.pmcid PMC5067594
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Economía y Competitividad (MINECO)


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