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Identification of ovule and seed genes from Citrus clementina

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Identification of ovule and seed genes from Citrus clementina

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dc.contributor.author García Lor, Andrés es_ES
dc.contributor.author Garcia Martinez, Jose L es_ES
dc.contributor.author Perez Amador, Miguel Angel es_ES
dc.coverage.spatial east=-0.3954708999999639; north=39.58827040000001; name=46113 Montcada, València, Espanya es_ES
dc.date.accessioned 2017-06-22T11:58:10Z
dc.date.available 2017-06-22T11:58:10Z
dc.date.issued 2012-04
dc.identifier.issn 1614-2942
dc.identifier.uri http://hdl.handle.net/10251/83446
dc.description.abstract [EN] Seedlessness is a highly desirable trait in fresh fruit. Citrus varieties, such as Clementine mandarin and other related species, show parthenocarpic fruit development without seeds due to self-incompatibility. In spite of that, these fruits frequently contain seeds as a result of cross-pollination by insects with compatible pollen from other citrus cultivars grown nearby. To solve this problem using a biotechnological approach we aim at the destruction of ovules and/or seeds by directing the expression of a toxic gene using the promoter of an ovule and/or seed specific gene. With the purpose of isolating this kind of genes we constructed two cDNA libraries from ovules and seeds at different developmental stages of the Clementine mandarin (Citrus clementina cv. Clemenules). A total of 1,014 ESTs from the ovule library and 1,042 ESTs from the seed library were generated, with a novelty percentage of 27% and 36% among the Spanish Citrus Functional Genomic Project (CFGP) ESTs database, respectively. Quantitative PCR analysis confirmed nearly specific expression in ovule and/or seed of two genes, TRANSPARENT TESTA16 (CcTT16) and TRANSPARENT TESTA7 (CcTT7). Expression of these two genes is restricted to early seed development, and is localized in the embryo sac and endothelium. The promoters of those genes may be useful to genetically engineer citrus species to avoid seed formation in fruits of commercial varieties. es_ES
dc.description.sponsorship The authors thank Dr. L. Navarro and J. Juarez, from the Instituto Valenciano de Investigaciones Agrarias (Generalitat Valenciana), for the use of the Clementine orchard and Fortune pollen; Dr. J Forment and the Genomics Facility at the IBMCP for sequence analysis and maintenance of to the CFGP database; and Drs. F Tadeo and J Carbonell for critical reading of the manuscript. This work was supported by grants from the Conselleria de Agricultura, Pesca y Alimentacion (Generalitat Valenciana) and Spanish Ministerio de Ciencia y Tecnologia (research grant GEN2001-4885-C05). A.G-L. received a PhD fellowship from the Conselleria de Agricultura, Pesca y Alimentacion. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Tree Genetics and Genomes es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Ovule es_ES
dc.subject Seed es_ES
dc.subject EST. es_ES
dc.subject Citrus. es_ES
dc.subject Clementine (Citrus clementina) es_ES
dc.title Identification of ovule and seed genes from Citrus clementina es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11295-011-0435-x
dc.relation.projectID info:eu-repo/grantAgreement/MICYT//GEN2001-4885-C05/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation García Lor, A.; Garcia Martinez, JL.; Perez Amador, MA. (2012). Identification of ovule and seed genes from Citrus clementina. Tree Genetics and Genomes. 8(2):227-235. https://doi.org/10.1007/s11295-011-0435-x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s11295-011-0435-x es_ES
dc.description.upvformatpinicio 227 es_ES
dc.description.upvformatpfin 235 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 232915 es_ES
dc.contributor.funder Ministerio de Ciencia y Tecnología es_ES
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