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A non-coding RNA induces changes in dynamic DNA methylation of ribosomal RNA genes in host plants

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A non-coding RNA induces changes in dynamic DNA methylation of ribosomal RNA genes in host plants

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dc.contributor.author Martínez Arias, Germán Eugenio es_ES
dc.contributor.author Castellano Pérez, Mayte es_ES
dc.contributor.author Tortosa-Viqueira, Maria es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Gomez, Gustavo Germán es_ES
dc.date.accessioned 2017-06-23T12:25:44Z
dc.date.available 2017-06-23T12:25:44Z
dc.date.issued 2014-02
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10251/83564
dc.description.abstract [EN] Viroids are plant-pathogenic non-coding RNAs able to interfere with as yet poorly known host-regulatory pathways and to cause alterations recognized as diseases. The way in which these RNAs coerce the host to express symptoms remains to be totally deciphered. In recent years, diverse studies have proposed a close interplay between viroid-induced pathogenesis and RNA silencing, supporting the belief that viroid-derived small RNAs mediate the post-transcriptional cleavage of endogenous mRNAs by acting as elicitors of symptoms expression. Although the evidence supporting the role of viroid-derived small RNAs in pathogenesis is robust, the possibility that this phenomenon can be a more complex process, also involving viroid-induced alterations in plant gene expression at transcriptional levels, has been considered. Here we show that plants infected with the 'Hop stunt viroid' accumulate high levels of sRNAs derived from ribosomal transcripts. This effect was correlated with an increase in the transcription of ribosomal RNA (rRNA) precursors during infection. We observed that the transcriptional reactivation of rRNA genes correlates with a modification of DNA methylation in their promoter region and revealed that some rRNA genes are demethylated and transcriptionally reactivated during infection. This study reports a previously unknown mechanism associated with viroid (or any other pathogenic RNA) infection in plants providing new insights into aspects of host alterations induced by the viroid infectious cycle. es_ES
dc.description.sponsorship The Spanish granting agency Direccion General de Investigacion Cientifica [BIO2011-25018 to V.P.] and from the Prometeo program [2011/003] from the Generalitat Valenciana. GM is the recipient of a Marie Curie IOF fellowship. Funding for open access charge: Direccion General de Investigacion Cientifica [BIO2011-25018]. en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP) es_ES
dc.relation.ispartof Nucleic Acids Research es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject Non-coding RNA es_ES
dc.subject DNA methylation es_ES
dc.subject Ribosoal RNA genes es_ES
dc.subject Plants es_ES
dc.title A non-coding RNA induces changes in dynamic DNA methylation of ribosomal RNA genes in host plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/nar/gkt968
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-25018/ES/TRAFICO INTRACELULAR, INTERCELULAR Y VASCULAR DE RNAS Y PROTEINAS VIRALES Y SUBVIRALES EN PLANTAS¿/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2009%2F125/ES/Efecto de la crioconservación de embriones sobre el desarrollo y el re-establecimiento de poblaciones/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Martínez Arias, GE.; Castellano Pérez, M.; Tortosa-Viqueira, M.; Pallás Benet, V.; Gomez, GG. (2014). A non-coding RNA induces changes in dynamic DNA methylation of ribosomal RNA genes in host plants. Nucleic Acids Research. 42(3):1553-1562. https://doi.org/10.1093/nar/gkt968 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1093/nar/gkt968 es_ES
dc.description.upvformatpinicio 1553 es_ES
dc.description.upvformatpfin 1562 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 42 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 256837 es_ES
dc.identifier.eissn 1362-4962
dc.identifier.pmcid PMC3919566
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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