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First detection of Grapevine rupestris stem pitting-associated virus and Grapevine rupestris vein feathering virus, and new phylogenetic groups for Grapevine fleck virus and Hop stunt viroid isolates, revealed from grapevine field surveys in Spain

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First detection of Grapevine rupestris stem pitting-associated virus and Grapevine rupestris vein feathering virus, and new phylogenetic groups for Grapevine fleck virus and Hop stunt viroid isolates, revealed from grapevine field surveys in Spain

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dc.contributor.author Fiore, Nicola es_ES
dc.contributor.author Zamorano, Alan es_ES
dc.contributor.author Sánchez Diana, Nicolás es_ES
dc.contributor.author González, Ximena es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Sanchez Navarro, Jesus Angel es_ES
dc.date.accessioned 2017-06-27T12:21:39Z
dc.date.available 2017-06-27T12:21:39Z
dc.date.issued 2016
dc.identifier.issn 0031-9465
dc.identifier.uri http://hdl.handle.net/10251/83825
dc.description.abstract [EN] Evaluation of the prevalence of virus and viroid infections was conducted in a grapevine field collection in Valencia, Spain. Samples of autochthonous and traditional grapevine cultivars were collected during November 2011 and tested for the presence of fourteen viruses and five viroids, using RT-PCR. The prevalent viruses were Grapevine rupestris stem pitting-associated virus (GRSPaV: 49% infected samples) and Grapevine leafroll-associated virus 2 (GLRaV-2: 15% of samples). GLRaV-1, GLRaV-3, GLRaV-4 (variants 4 and 5), Grapevine fanleaf virus, Grapevine fleck virus (GFkV), Grapevine rupestris vein feathering virus (GRVFV) and Grapevine virus A were also detected. Hop stunt viroid (HSVd: 92% of plants infected) and Grapevine yellow speckle viroid 1 (6% of plants) were also detected. Mixed infections with two, and up to six different viruses and/or viroids were common. Only five samples (4%) were free from 19 pathogens tested. This is the first report of GLRaV-4 (variants 4 and 5) in the Valencia region of Spain, and the first record of GRSPaV and GRVFV in this country. Phylogenetic analyses performed with the sequences of these viruses showed that the Spanish isolates of GLRaV-4, GFkV and HSVd belong to new phylogenetic groups. es_ES
dc.description.sponsorship This study was supported by Projects Consejo Superior de Investigaciones Científicas CSIC (2010CL0021) and BIO2011-25018 from the Spanish MINECO / UNIVERSIDAD DE CHILE 04/11-2 and 2010CL0021
dc.language Inglés es_ES
dc.publisher Firenze University Press es_ES
dc.relation CSIC/2010CL0021 es_ES
dc.relation MINECO/BIO2011-25018 es_ES
dc.relation UCHILE/04/11-2
dc.relation UCHILE/2010CL0021
dc.relation.ispartof Phytopathologia Mediterranea es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Detection, reverse transcription-polymerase chain reaction, sequencing, phylogenetic analysis es_ES
dc.subject Detection es_ES
dc.subject Reverse transcription-polymerase chain reaction es_ES
dc.subject Sequencing es_ES
dc.subject Phylogenetic analysis es_ES
dc.subject.classification TECNOLOGIA DE ALIMENTOS es_ES
dc.title First detection of Grapevine rupestris stem pitting-associated virus and Grapevine rupestris vein feathering virus, and new phylogenetic groups for Grapevine fleck virus and Hop stunt viroid isolates, revealed from grapevine field surveys in Spain es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.14601/Phytopathol_Mediterr-15875
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Fiore, N.; Zamorano, A.; Sánchez Diana, N.; González, X.; Pallás Benet, V.; Sanchez Navarro, JA. (2016). First detection of Grapevine rupestris stem pitting-associated virus and Grapevine rupestris vein feathering virus, and new phylogenetic groups for Grapevine fleck virus and Hop stunt viroid isolates, revealed from grapevine field surveys in Spain. Phytopathologia Mediterranea. 55(2):225-238. doi:10.14601/Phytopathol_Mediterr-15875 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.14601/Phytopathol_Mediterr-15875 es_ES
dc.description.upvformatpinicio 225 es_ES
dc.description.upvformatpfin 238 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 55 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 323854 es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Universidad de Chile


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