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PsPMEP, a pollen specific pectin methylesterase of pea (Pisum sativum L.)

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PsPMEP, a pollen specific pectin methylesterase of pea (Pisum sativum L.)

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dc.contributor.author Gómez Jiménez, Maria Dolores es_ES
dc.contributor.author Renau Morata, Begoña es_ES
dc.contributor.author Roque Mesa, Edelin Marta es_ES
dc.contributor.author Polaina, J es_ES
dc.contributor.author Beltran Porter, Jose Pio es_ES
dc.contributor.author Cañas Clemente, Luís Antonio es_ES
dc.date.accessioned 2017-07-06T06:19:38Z
dc.date.available 2017-07-06T06:19:38Z
dc.date.issued 2013-09
dc.identifier.issn 2194-7953
dc.identifier.uri http://hdl.handle.net/10251/84525
dc.description.abstract [EN] Pectin methylesterases (PMEs) are a family of enzymes involved in plant reproductive processes such as pollen development and pollen tube growth. We have isolated and characterized PsPMEP, a pea (Pisum sativum L.) pollen-specific gene that encodes a protein with homology to PMEs. Sequence analysis showed that PsPMEP belongs to group 2 PMEs, which are characterized by the presence of a processable amino-terminal PME inhibitor domain followed by the catalytic PME domain. Moreover, PsPMEP contains several motifs highly conserved among PMEs with the essential amino acid residues involved in enzyme substrate binding and catalysis. Northern blot and in situ hybridization analyses showed that PsPMEP is expressed in pollen grains from 4 days before anthesis till anther dehiscence and in pollinated carpels. In the PsPMEP promoter region, we have identified several conserved cis-regulatory elements that have been associated with gene pollen-specific expression. Expression analysis of PsPMEP promoter fused to the uidA reporter gene in Arabidopsis thaliana plants showed a similar expression pattern when compared with pea, indicating that this promoter is also functional in a non-leguminous plant. GUS expression was detected in mature pollen grains, during pollen germination, during pollen tube elongation along the transmitting tract, and when the pollen tube reaches the embryo sac in the ovule. es_ES
dc.description.sponsorship This work was funded by grants BIO2006-09374 and BIO2009-08134 from the Spanish Ministry of Science and Innovation (MICINN). The collaboration and assistance of Julia Marin-Navarro in the catalytic activity assays of PsPMEP in yeast and Rafael Martinez-Pardo in the greenhouse is gratefully acknowledged. We would like to thank the HAPRECI consortium (COST Action FA0903) to bring us the opportunity to collaborate with other European research groups working in the field of Plant Reproduction and to select our manuscript to be published in this special issue.
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Plant Reproduction es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Pectin methylesterase es_ES
dc.subject Pea (Pisum sativum L.) es_ES
dc.subject Pollen-specific es_ES
dc.subject PMEI es_ES
dc.subject Gene promoter es_ES
dc.subject Pollen tube growth es_ES
dc.subject Arabidopsis es_ES
dc.title PsPMEP, a pollen specific pectin methylesterase of pea (Pisum sativum L.) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00497-013-0220-0
dc.relation.projectID info:eu-repo/grantAgreement/MEC//BIO2006-09374/ES/ANALISIS GENETICO Y FUNCIONAL DEL DESARROLLO FLORAL EN MEDICAGO TRUNCATULA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2009-08134/ES/Mejora Del Valor Nutritivo De La Alfalfa (Medicago Sativa L.) Mediante Ingenieria Genetica/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.description.bibliographicCitation Gómez Jiménez, MD.; Renau Morata, B.; Roque Mesa, EM.; Polaina, J.; Beltran Porter, JP.; Cañas Clemente, LA. (2013). PsPMEP, a pollen specific pectin methylesterase of pea (Pisum sativum L.). Plant Reproduction. 26(3):245-254. https://doi.org/10.1007/s00497-013-0220-0 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s00497-013-0220-0 es_ES
dc.description.upvformatpinicio 245 es_ES
dc.description.upvformatpfin 254 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 259583 es_ES
dc.identifier.pmid 23839307
dc.contributor.funder Ministerio de Educación y Ciencia
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