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dc.contributor.author | Soler, Nuria | es_ES |
dc.contributor.author | Plomer Sáez, Montserrat | es_ES |
dc.contributor.author | Fagoaga García, Carmen Concepción | es_ES |
dc.contributor.author | Moreno, Pedro | es_ES |
dc.contributor.author | Navarro, Luis | es_ES |
dc.contributor.author | Flores Pedauye, Ricardo | es_ES |
dc.contributor.author | PEÑA GARCIA, LEANDRO | es_ES |
dc.date.accessioned | 2017-07-18T11:28:37Z | |
dc.date.available | 2017-07-18T11:28:37Z | |
dc.date.issued | 2012-06 | |
dc.identifier.issn | 1467-7644 | |
dc.identifier.uri | http://hdl.handle.net/10251/85385 | |
dc.description.abstract | [EN] Citrus tristeza virus (CTV), the causal agent of the most devastating viral disease of citrus, has evolved three silencing suppressor proteins acting at intra- (p23 and p20) and/or intercellular level (p20 and p25) to overcome host antiviral defence. Previously, we showed that Mexican lime transformed with an intron-hairpin construct including part of the gene p23 and the adjacent 3' untranslated region displays partial resistance to CTV, with a fraction of the propagations from some transgenic lines remaining uninfected. Here, we transformed Mexican lime with an intron-hairpin vector carrying full-length, untranslatable versions of the genes p25, p20 and p23 from CTV strain T36 to silence the expression of these critical genes in CTV-infected cells. Three transgenic lines presented complete resistance to viral infection, with all their propagations remaining symptomless and virus-free after graft inoculation with CTV-T36, either in the nontransgenic rootstock or in the transgenic scion. Accumulation of transgene-derived siRNAs was necessary but not sufficient for CTV resistance. Inoculation with a divergent CTV strain led to partially breaking the resistance, thus showing the role of sequence identity in the underlying mechanism. Our results are a step forward to developing transgenic resistance to CTV and also show that targeting simultaneously by RNA interference (RNAi) the three viral silencing suppressors appears critical for this purpose, although the involvement of concurrent RNAi mechanisms cannot be excluded. | es_ES |
dc.description.sponsorship | We thank J.E. Peris for his excellent technical assistance and Dr. W.O. Dawson (University of Florida, C.R.E.C., Lake Alfred, FL, USA) for providing the GFP-tagged CTV strain CTV947R-GFP. N.S. was supported by a PhD fellowship from the IVIA. C.F. is recipient of a postdoctoral Ramon y Cajal contract from the Ministerio de Ciencia e Innovacion (MICINN). This research was supported by grants AGL2009-08052, co-financed by Fondo Europeo de Desarrollo Regional-MICINN, and Prometeo/2008/121 from the Generalitat Valenciana. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Wiley Open Access | es_ES |
dc.relation.ispartof | Plant Biotechnology Journal | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Antiviral defence | es_ES |
dc.subject | Citrus | es_ES |
dc.subject | RNA interference | es_ES |
dc.subject | Silencing suppressor | es_ES |
dc.subject | Transgenic citrus | es_ES |
dc.subject | Virus resistance | es_ES |
dc.title | Transformation of Mexican lime with an intron-hairpin construct expressing untranslatable versions of the genes coding for the three silencing suppressors of Citrus tristeza virus confers complete resistance to the virus | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/j.1467-7652.2012.00691.x | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2009-08052/ES/Mejora Genetica De La Calidad Y De La Respuesta A Estreses Bioticos De Los Citricos Mediante Ingenieria Genetica/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO08%2F2008%2F121/ES/Biotecnología de cítricos/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana | es_ES |
dc.description.bibliographicCitation | Soler, N.; Plomer Sáez, M.; Fagoaga García, CC.; Moreno, P.; Navarro, L.; Flores Pedauye, R.; Peña Garcia, L. (2012). Transformation of Mexican lime with an intron-hairpin construct expressing untranslatable versions of the genes coding for the three silencing suppressors of Citrus tristeza virus confers complete resistance to the virus. Plant Biotechnology Journal. 10(5):597-608. https://doi.org/10.1111/j.1467-7652.2012.00691.x | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1111/j.1467-7652.2012.00691.x | es_ES |
dc.description.upvformatpinicio | 597 | es_ES |
dc.description.upvformatpfin | 608 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 10 | es_ES |
dc.description.issue | 5 | es_ES |
dc.relation.senia | 233250 | es_ES |
dc.identifier.pmid | 22405601 | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |