Minoia, S.; Navarro, B.; Delgado Villar, SG.; Di Serio, F.; Flores Pedauye, R. (2015). Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo. Nucleic Acids Research. 43(4):2313-2325. doi:10.1093/nar/gkv034
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/85435
Title:
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Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo
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Author:
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Minoia, Sofia
Navarro, B
Delgado Villar, Sonia Guadalupe
Di Serio, Francesco
Flores Pedauye, Ricardo
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UPV Unit:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
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Issued date:
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Abstract:
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[EN] While biogenesis of viroid RNAs is well-known, how they decay is restricted to data involving host RNA silencing. Here we report an alternative degradation pathway operating on potato spindle tuber viroid (PSTVd), the ...[+]
[EN] While biogenesis of viroid RNAs is well-known, how they decay is restricted to data involving host RNA silencing. Here we report an alternative degradation pathway operating on potato spindle tuber viroid (PSTVd), the type species of nuclear-replicating viroids (family Pospiviroidae). Northern-blot hybridizations with full-and partial-length probes revealed a set of PSTVd (+) subgenomic (sg) RNAs in early-infected eggplant, some partially overlapping and reaching levels comparable to those of the genomic circular and linear forms. Part of the PSTVd (+) sgRNAs were also observed in Nicotiana benthamiana (specifically in the nuclei) and tomato, wherein they have been overlooked due to their low accumulation. Primer extensions of representative (+) sgRNAs failed to detect a common 5' terminus, excluding that they could result from aborted transcription initiated at one specific site. Supporting this view, 5'- and 3'-RACE indicated that the (+) sgRNAs have 5'-OH and 3'-P termini most likely generated by RNase-mediated endonucleolytic cleavage of longer precursors. These approaches also unveiled PSTVd (-) sgRNAs with features similar to their (+) counterparts. Our results provide a mechanistic insight on how viroid decay may proceed in vivo during replication, and suggest that synthesis and decay of PSTVd strands might be coupled as in mRNA.
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Subjects:
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Nicotiana benthamiana
,
Systemic trafficking
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hammerhead ribozymes
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Secondary structure
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Situ hybridization
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Host interactions
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Rolling circle
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Self cleavage
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Replication
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Sequence
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Copyrigths:
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Reconocimiento - No comercial (by-nc)
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Source:
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Nucleic Acids Research. (issn:
0305-1048
) (eissn:
1362-4962
)
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DOI:
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10.1093/nar/gkv034
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Publisher:
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Oxford University Press (OUP)
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Publisher version:
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http://doi.org/10.1093/nar/gkv034
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Project ID:
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Ministerio de Economía y Competitividad (MINECO), Spain [BFU2011-28443]
Ministero dell'Economia e Finanze Italiano (CISIA) [Legge 191/2009]
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Thanks:
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Ministerio de Economia y Competitividad (MINECO) [BFU2011-28443 to R. F. laboratory]; MINECO [to S.M. and S.D.]. Funding for open access charge: MINECO (BFU2011-28443 to R. F.); Ministero dell'Economia e Finanze Italiano ...[+]
Ministerio de Economia y Competitividad (MINECO) [BFU2011-28443 to R. F. laboratory]; MINECO [to S.M. and S.D.]. Funding for open access charge: MINECO (BFU2011-28443 to R. F.); Ministero dell'Economia e Finanze Italiano to the CNR (CISIA, Legge 191/2009 to B.N. and F.D.S.).
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Type:
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Artículo
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